Resumen de Matrix production of smooth muscle cells from rat aorta in vitro

B. Stieme, G. Springmeier, L. El Jarad, C. Schroter Kermani

• Immunofluorescence microscopic methods served to demonstrate the production of the following matrix components in cultures of vascular smooth muscle cells from rat aorta: fibronectin; nidogen; heparan sulphate-proteoglycan (HS-PG); laminin; and collagen types 1, 111, IV, V, and VI. A time-dependence of synthesis and secretion could be shown for a number of components of the extracellular matrix (ECM), such as laminin. The results revealed the following estimated quantitative differences of the collagen types: type 1 > type 111 > types V and VI. A filamentouslfibrillar matrix and also occasionally a typical basal lamina could be demonstrated electron microscopically around the smooth muscle cells.