Bacterial Analogs of Plant Tetrahydropyridine Alkaloids Mediate Microbial Interactions in a Rhizosphere Model System

• Gabriel L. Lozano ^[5] ; Hyun Bong Park ^[1] ; Juan I. Bravo ^[5] ; Eric A. Armstrong ^[2] ; John M. Denu ^[2] ; Eric V. Stabb ^[3] ; Nichole A. Broderick ^[4] ; Jason M. Crawford ^[1] ; Jo Handelsman ^[5]
  1. [1] Yale University

     Yale University

     Town of New Haven, Estados Unidos

     
  2. [2] University of Wisconsin–Madison

     University of Wisconsin–Madison

     City of Madison, Estados Unidos

     
  3. [3] University of Georgia

     University of Georgia

     Estados Unidos

     
  4. [4] University of Connecticut

     University of Connecticut

     Town of Mansfield, Estados Unidos

     
  5. [5] a Wisconsin Institute for Discovery and Department of Plant Pathology, University of Wisconsin–Madison, Madison, Wisconsin, USA; b Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, Connecticut, USA

  Mostrar afiliaciones +
• Localización: Applied and Environmental Microbiology, ISSN 0099-2240, Vol. 85, Nº 10, 2019
• Idioma: inglés
• Enlaces
  • Texto completo
• Resumen

  • The microbiomes of plants are critical to host physiology and development. Microbes are attracted to the rhizosphere due to massive secretion of plant photosynthates from roots. Microorganisms that successfully join the rhizosphere community from bulk soil have access to more abundant and diverse molecules, producing a highly competitive and selective environment. In the rhizosphere, as in other microbiomes, little is known about the genetic basis for individual species’ behaviors within the community. In this study, we characterized competition between Pseudomonas koreensis and Flavobacterium johnsoniae, two common rhizosphere inhabitants. We identified a widespread gene cluster in several Pseudomonas spp. that is necessary for the production of a novel family of tetrahydropyridine alkaloids that are structural analogs of plant alkaloids. We expand the known repertoire of antibiotics produced by Pseudomonas in the rhizosphere and demonstrate the role of the metabolites in interactions with other rhizosphere bacteria.