Expression of γ-tubulin in non-small cell lung cancer and effect on patient survival

• Nicoletta F. Maounis ^[1] ; Eduarda Dráberová ^[2] ; Nikos Trakas ^[3] ; Maria Chorti ^[3] ; Dimitra Riga ^[5] ; Kimon Tzannis ^[4] ; Meletis Kanakis ^[3] ; Kirtanaa Voralu ^[6] ; Eleni Ellina ^[3] ; Eleni Mahera ^[5] ; Maria Demonakou ^[3] ; Achilleas Lioulias ^[3] ; Pavel Dráber ^[2] ; Katsetos, Christos D. ^[1]
  1. [1] Drexel University

     Drexel University

     City of Philadelphia, Estados Unidos

     
  2. [2] Academy of Sciences of the Czech Republic

     Academy of Sciences of the Czech Republic

     Chequia

     
  3. [3] Sismanoglio General Hospital

     Sismanoglio General Hospital

     Dimos Athens, Grecia

     
  4. [4] National and Kapodistrian University of Athens

     National and Kapodistrian University of Athens

     Dimos Athens, Grecia

     
  5. [5] “KAT” General Hospital, Attica, Greece
  6. [6] Houston Methodist Hospital, Houston, Texas

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• Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 34, Nº. 1, 2019, págs. 81-90
• Idioma: inglés
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• Resumen

  • Introduction. It has been reported that overexpression and altered compartmentalization of γ- tubulin may contribute to tumorigenesis and tumor aggressiveness in a variety of human malignancies. We have shown that γ-tubulin expression and cellular distribution pattern is also altered in non-small cell lung cancer (NSCLC) (Histol. Histopathol. 2012; 27: 1183- 1194). In the present study we examined the relationship between γ-tubulin expression and patient overall survival (OS). Material and methods. Immunohistochemistry was performed, with well-characterized anti-γ-tubulin antibodies, on 109 formalin-fixed, paraffin-embedded NSCLC specimens (p-TNM stage IIII).

    γ-Tubulin labeling indexes (LIs) were determined, and the association of γ-tubulin expression with clinicopathological parameters was evaluated. To analyze OS rates according to γ-tubulin LIs, patients were categorized into three groups: those with low (0- 30%), intermediate (31-69%) or high (70-100%) γ- tubulin LI. Association of clinicopathological parameters and γ-tubulin with survival were examined using univariate and multivariate Cox regression analysis.

    Results. No statistically significant association was seen between γ-tubulin overexpression and histological type, tumor differentiation, p-TNM stage and adenocarcinoma subtyping. Longer survival was observed in the high γ- tubulin LI group of patients with p-TNM stages II+III when compared to intermediate or low γ-tubulin LI groups, but the difference was not statistically significant (p=0.066). On the other hand, when combined low and intermediate γ-tubulin LI groups (p-TNM stages II+III) where compared to high γ-tubulin LI group, statistically significant longer survival was observed in high γ- tubulin group (p=0.021). Conclusion. Our findings suggest that level of γ-tubulin expression may have an impact on patient survival at more advanced NSCLC stages.