Effectiveness of culture media on the success of embryo rescue among different crosses of seedless grapes

• Autores: H. Wang, Y. Z Hao, R. Yang, F.-L. Wang, J. Lu
• Localización: X International Conference on Grapevine Breeding and Genetics / coord. por Bruce Irving Reisch, J. P. Londo, 2014, ISBN 9789462610347, págs. 259-263
• Idioma: inglés
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• Resumen

  • Effects of culture media on the success of embryo rescue were tested among three different cross combinations of seedless grapes. ER, N&N, WP and ½ MS media were used. The best medium for ovule development was ER + 6-BA 0.5 mg/L among all 3 cross combinations. For embryo germination and plantlet formation, the success rate varied among different cross combinations. The best media for seedling establishment in ?Flame Seedless? × ?Centennial?, for example, were 1/2 MS for embryo germination and 1/2 MS + IBA 0.1 mg/L for seedling growth. The best media for ?Otilia? × ?Venus? were 1/2 MS + IAA 0.1 mg/L for embryo germination, 1/2 MS + IAA 0.1 mg/L for seedling growth, and half strength B5 + IAA 0.125 mg/L for subculture, respectively. The best media for ?Venus? × ?Himrod? were 1/2 MS for embryo germination, WP + 6-BA 0.1 mg/L for seedling growth, half strength of B5 + IAA 0.125 mg/L for subculture, respectively.