Modulatory role of IL10 in endothelial cell damage and platelet adhesion

M.J. Gimeno ^[1] ; G. Pascual ^[1] ; N. García-Honduvilla ^[1] ; A. Prieto ^[1] ; M. Alvarez de Mon ^[2] ; J.M. Bellón ^[1] ; J. Buján ^[1]
1. [1] Universidad de Alcalá
  
  Universidad de Alcalá
  
  Alcalá de Henares, España
2. [2] University Hospital Principe de Asturias
Localización: Histology and histopathology: cellular and molecular biology, ISSN-e 1699-5848, ISSN 0213-3911, Vol. 18, Nº. 3, 2003, págs. 695-702
Idioma: inglés
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Resumen
- This study explores the possibility of a regulatory role for cytokine IL-10 in platelet aggregation as an active vascular repair mechanism. Endothelial cells from human umbilical cord vein were cultured in the presence of different IL-10 concentrations (0-100 ng/ml). Platelet-rich plasma was then added to these cultures and allowed to act for 30 minutes. To rule out blood plasma involvement, washed platelets were also incubated with IL-10 (0-100 ng/ml). Changes in endothelial cell morphology were observed depending on the IL-10 concentration used; apoptotic cells appearing at the highest IL-10 concentration. Greatest platelet adhesion was noted at the highest IL-10 concentration. It was concluded that, in this in vitro model, low IL-10 levels do not affect cell viability or the pattern of platelet adhesion, but at high doses, this cytokine induces cell death and enhances platelet deposition.