Comparative effect of the fungicide Prochloraz-Mn on Agaricus bisporus vegetative-mycelium and fruit-body cell walls

• Dolores Bernardo ^[1] ; Amelia Pérez Cabo ^[1] ; Monique Novaes-Ledieu ^[1] ; José Pardo ^[2] ; Concepción García Mendoza ^[1]
  1. [1] Biological Research Center, CSIC, Madrid, Spain
  2. [2] Center for Research, Experimentation and Services of Mushrooms, Quintanar del Rey, Cuenca, Spain
• Localización: International microbiology: official journal of the Spanish Society for Microbiology, ISSN 1139-6709, Vol. 7, Nº. 4, 2004, págs. 277-281
• Idioma: inglés
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• Resumen

  • Fungicides to control mycopathogens of commercial Agaricus bisporus, a mushroom cultivated for human consumption, are a major field of study, since these chemicals are toxic to both the host and its fungal parasites. The fungicide Prochloraz-Mn, used at its LD50 for A. bisporus, partially inhibited protein biosynthesis in the vegetative mycelial cell walls of this mushroom and caused significant changes in cell-wall polysaccharide structure, as deduced by methylation analysis and gas liquid chromatography-mass spectrometry (GLC-MS). Furthermore, the aggregated mycelial walls showed distinct alterations in their overall chemical composition following the administration of Prochloraz-Mn at the LD50 and the LD50 ×1000. As expected, GLC-MS studies indicated that the latter dose caused more appreciable differences in polysaccharide structure. The decrease in mushroom crop yields obtained from industrial cultures treated with Prochloraz-Mn to control V. fungicola infection depended on the dose of the fungicide employed, whereas fruit-body morphology was only slightly affected at the highest Prochloraz-Mn concentration used. [Int Microbiol 2004; 7(4):277-281]