Molecular genetic study of novel biomarkers for early diagnosis of oral squamous cell carcinoma
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Kim Yong-Deok [1] ; Jeon Eun-Hyoung [1] ; Kim Yeon-Sun [1] ; Pang Kang-Mi [2] ; Lee Jin-Yong [3] ; Cho Sung-Hwan [1] ; Kim Tae-Yun [1] ; Park Tae-Sung [1] ; Kim Soung-Min [1] ; Kim Myung-Jin [1] ; Lee Jong-Ho [1]
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[1] Seoul National University
Seoul National University
Corea del Sur
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[2] Ajou University
Ajou University
Corea del Sur
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[3] Korea University
Korea University
Corea del Sur
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- Localización: Medicina oral, patología oral y cirugía bucal. Ed. inglesa, ISSN-e 1698-6946, Vol. 20, Nº. 2 (Marzo), 2015
- Idioma: inglés
- Enlaces
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Resumen
Objectives: Early detection and treatment of an oral squamous cell carcinoma (OSCC) is critical because of its rapid growth, frequent lymph-node metastasis, and poor prognosis. However, no clinically-valuable methods of early diagnosis exist, and genetic analysis of OSCCs has yielded no biomarkers.
Study D esign: We investigated the expression of genes associated with inflammation in OSCCs via a quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis of microarray data. Tumor and normal tissues from five patients with an OSCC were used for microarray analysis. Differentially-expressed genes, identified using permutation, local pooled error (LPE), t-tests, and significance analysis of microarrays (SAM), were selected as candidate genetic markers.
Results: Two groups corresponding to tissue identity were evident, implying that their differentially-expressed genes represented biological differences between tissues. Fifteen genes were identified using the Student’s paired t-test ( p< 0.05) and the SAM, with a false discovery rate of less than 0.02. Based on gene expression, these 15 genes can be used to classify an OSCC. A genetic analysis of functional networks and ontologies, validated by using a qRT-PCR analysis of the tissue samples, identified four genes, ADAM15, CDC7, IL12RB2 and TNFRSF8, that demonstrated excellent concordance with the microarray data.
Conclusions: Our study demonstrated that four genes (ADAM15, CDC7, IL12RB2 and TNFRSF8) had potential as novel biomarkers for the diagnosis and the treatment of an OSCC.
