New Biomarker Evidence of Oxidative DNA Damage in Whole Saliva From Clinically Healthy and Periodontally Diseased Individuals

• Autores: Masatoshi Takane, Naoyuki Sugano, Hiroyasu Iwasaki, Yoshihiro Iwano, Noritaka Shimizu, Koichi Ito
• Localización: Journal of periodontology, ISSN 0022-3492, Vol. 73, Nº. 5, 2002, págs. 551-554
• Idioma: inglés
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• Resumen

  • Background: There is an increasing body of evidence implicating reactive oxygen species in the pathogenesis of periodontal tissue destruction. 8-hydroxy-deoxyguanosine (8-OHdG) is one of the most commonly used markers to evaluate oxidative damage in a number of disorders including chronic inflammatory diseases. The aim of the present study was to evaluate 8-OHdG levels in whole saliva of patients with periodontitis and to assess the changes after initial treatment.

    Methods: Saliva samples were collected from 78 patients with untreated periodontitis and 17 healthy control subjects. Clinical parameters and levels of 8-OHdG were assessed first to establish a baseline and again after initial periodontal treatment from 15 patients. 8-OHdG levels were determined by enzymelinked immunosorbent assay.

    Results: The mean value of 8-OHdG in the saliva of periodontally diseased subjects, 4.28 ± 0.10 ng/ml, was significantly higher (P <0.01) than that of clinically healthy subjects (1.56 ± 0.10 ng/ml). A significant decrease in salivary 8-OHdG was observed after therapy (P <0.01).

    Conclusion: In the present study, we evaluated for the first time 8-OHdG levels in whole saliva of patients with periodontitis and assessed changes after initial periodontal treatment. Our study indicated that 8-OHdG levels in saliva appear to reflect the status of periodontal health. J Periodontol 2002;73:551-554.