Effects of Er:YAG Laser and Ultrasonic Treatment on Fibroblast Attachment to Root Surfaces: An In Vitro Study Roberto Crespi,* George E. Romanos,† Clara Cassinelli,‡ and Enrico Gherlone* *Dental School, University San Raffaele, Milan, Italy.
†Department of Periodontology and Implant Dentistry, New York University, New York, NY.
‡NobilBioricerche, Villafranca d'Asti, Italy.
Correspondence: Dr. Roberto Crespi, Via Cadore N9, 21052 Busto Arsizio (Varese), Italy. E-mail: robcresp@libero.it.
Background: The aim of this study was to analyze the effects of erbium-doped:yttrium, aluminum, and garnet (Er:YAG) laser and ultrasonic treatment on fibroblast attachment to periodontally diseased root surfaces.
Methods: Thirty single-rooted human periodontally involved teeth were included in this study. A total of 60 specimens were obtained from all selected teeth and were randomly assigned to the following three groups: group A, untreated control group; group B, ultrasonic group; and group C, Er:YAG laser at 160 mJ/pulse at 10 Hz group. All of the specimens were incubated in petri dishes with fibroblast suspension and observed by scanning electron microscopy.
Results: Laser-treated specimens showed a significantly higher cell density number, with a mean ± SD of 3,720 ± 316 cells/mm2. The ultrasonically treated group showed a lower cell density number, with a mean ± SD of 658 ± 140 cells/mm2. The untreated control group showed the lowest cell density number, with a mean ± SD of 130 ± 80 cells/mm2. Differences between all groups were significant (P <0.0001).
Conclusion: The results of the study indicate that untreated control surfaces and ultrasonically treated surfaces exhibited a significantly lower number of attached cells compared to laser-treated specimens, which showed a significantly higher cell density number.
KEYWORDS: Cell adhesion, fibroblasts, lasers/therapeutic use, periodontal ligament, tooth root, ultrasonic therapy
© 2001-2024 Fundación Dialnet · Todos los derechos reservados