Resumen de Effect of a Hydroxyapatite Tricalcium Phosphate Alloplast on Osseous Repair in the Rat Calvarium
Kurt B. Fleckenstein, Michael F. Cuenin, Mark E. Peacock, Michael A. Billman, Gary D. Swiec, Thomas B. Buxton, Baldev B. Singh, James C. McPherson
Correspondence: Dr. Mark E. Peacock, 500 Scotts Way, Augusta, GA 30909. E-mail: mark.peacock@lnd.amedd.army.mil.
Background: Bony defects caused by periodontitis are often treated by regenerative therapy using autografts and/or allografts. Alloplasts, such as hydroxyapatite or ceramics, are also used as osteoconductive materials that serve as a scaffold for new bony ingrowth. The purpose of this study was to determine the effect of hydroxyapatite tricalcium phosphate (HA-TCP) on osseous repair in the rat calvarium.
Methods: Forty-four adult male Harlan Sprague-Dawley rats were assigned to one of four treatment groups: HA-TCP macroporous disk, HA-TCP microporous disk, HA-TCP granules, and demineralized freeze-dried bone (DFDB). The materials were placed into 8-mm calvarial critical-size defects (CSD). Calvariae were harvested at 10 weeks post-surgery and evaluated histomorphometrically.
Results: The DFDB group had significantly (P <0.05) more new bone formation (47%) than any other group. The HA-TCP macroporous disk group had significantly (P <0.05) more new bone formation (19.7%) than the HA-TCP microporous disk (8.5%) or HA-TCP granule (6.9%) groups.
Conclusions: The HA-TCP macroporous disk may elicit significant new bone formation due to its rigid space-maintaining scaffold and pore size for vascular ingrowth. It is well tolerated by host tissues and may be a suitable carrier for growth factors.
