Resumen de The Effect of 0.12% Chlorhexidine Gluconate Rinsing on Previously Plaque-Free and Plaque-Covered Surfaces: A Randomized, Controlled Clinical Trial

Raquel P. Antoniazzi, Cassiano Kuchenbecker Rösing, Fabrício B. Zanatta

• The Effect of 0.12% Chlorhexidine Gluconate Rinsing on Previously Plaque-Free and Plaque-Covered Surfaces: A Randomized, Controlled Clinical Trial Fabrício B. Zanatta,*† Raquel P. Antoniazzi,* and Cassiano K. Rösing* *Postgraduate Program in Dentistry, Lutheran University of Brazil, Canoas, RS, Brazil.

  †School of Dentistry, Franciscan University Center, Santa Maria, RS, Brazil.

  Correspondence: Dr. Fabrício B. Zanatta, Rua Humberto de Campos, 250/302, 97095-230, Santa Maria, RS, Brazil. Fax: 55-51-33468789; e-mail: fabriciobzanatta@yahoo.com.br.

  Background: Previous in vitro studies showed little bactericidal effect on structured oral biofilm after exposure to chlorhexidine (CHX). In vivo evidence of a CHX effect against structured biofilm is scarce. The purpose of this study was to compare the efficacy of 0.12% CHX gluconate on previously plaque-free and plaque-covered surfaces.

  Methods: This study had a single-masked, randomized split-mouth, 21-day experimental gingivitis design including 20 individuals who refrained from all mechanical plaque control methods for 25 days. On day 4 of plaque accumulation, the individuals had two randomized quadrants cleaned; the other two quadrants served as the plaque-covered surfaces. Also, on day 4, the individuals started rinsing with 0.12% CHX gluconate for 21 days. The Quigley and Hein plaque index (PI), gingival index (GI), and gingival crevicular fluid (GCF) volume were assessed at baseline and days 21 and 25. The PI also was assessed at days 4, 11, and 18.

  Results: Intergroup comparisons showed statistically higher PI throughout the study on the plaque-covered surfaces compared to the plaque-free surfaces. When the inflammatory response over time was analyzed, a statistically greater increase in GI (from 0.21 ± 0.02 to 0.93 ± 0.03 versus from 0.18 ± 0.01 to 0.52 ± 0.03 on plaque-covered and plaque-free surfaces, respectively) and GCF volumes (from 48.09 to 94.28 μl versus from 46.94 to 64.99 μl on plaque-covered and plaque-free surfaces, respectively) occurred on plaque-covered surfaces after 21 days of plaque accumulation.

  Conclusions: A 0.12% CHX gluconate mouthrinse had little antiplaque and antigingivitis effect on previously plaque-covered surfaces. These results confirm the diminished effect of CHX on structured biofilm and reinforce the necessity of biofilm disruption before the initiation of CHX mouthrinse.

  KEYWORDS: Biofilm, chlorhexidine, dental plaque, gingival crevicular fluid, gingivitis Cited by Joel M. Laudenbach and Ziv Simon. (2014) Common Dental and Periodontal Diseases. Medical Clinics of North America 98, 1239-1260.

  Online publication date: 1-Nov-2014.

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  Online publication date: 1-Aug-2014.

  CrossRef Oya Türkoğlu, Sema Becerik, Taina Tervahartiala, Timo Sorsa, Gül Atilla and Gülnur Emingil. (2014) The effect of adjunctive chlorhexidine mouthrinse on GCF MMP-8 and TIMP-1 levels in gingivitis: a randomized placebo-controlled study. BMC Oral Health 14, 55.

  Online Daljit Kapoor, Navjot Kaur and Tarun Nanda. (2011) Efficacy of two different concentrations of chlorhexidine mouth-rinse on plaque re-growth. Indian Journal of Dentistry 2, 11-15.

  Online publication date: 1-Jun-2011.

  CrossRef Magda Feres, Lauren Christine Gursky, Marcelo Faveri, Claudia Ota Tsuzuki and Luciene Cristina Figueiredo. (2009) Clinical and microbiological benefits of strict supragingival plaque control as part of the active phase of periodontal therapy. Journal of Clinical Periodontology 36:10.1111/cpe.2009.36.issue-10, 857-867.

  Online publication date: 1-Oct-2009.

  CrossRef Samer B. Khoury, Leslie Thomas, John D. Walters, John F. Sheridan and Binnaz Leblebicioglu. (2008) Early Wound Healing Following One-Stage Dental Implant Placement With and Without Antibiotic Prophylaxis: A Pilot Study. Journal of Periodontology 79:10, 1904-1912.

  Online publication date: 1-Oct-2008.