Desarrollo de un bioproceso industrial de la producción de ácido cítrico por aspergillus niger
- Autores: César Reyes, Luis López, Humberto Rubí, Octavio Ramírez
- Localización: Ciencias Naturales y Exactas Handbook: Congreso Interdisciplinario de Cuerpos Académicos / coord. por Marcos Rodríguez Sánchez, José Alfredo Aguirre Puente, 2013, ISBN 978-607-8324-06-4, págs. 137-151
- Idioma: español
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Resumen
Organic acids, such as citric acid, have increased their demand in the food industry and is one of the main metabolites produced by microorganisms in the Biotechnology industry.
There are several citric acid-producing microorganisms: bacteria, fungi and yeasts, but the most used is the filamentous fungus Aspergillus niger called because it is the most adaptable to grow on various types of substrates .. The main purpose is to mount a bioprocess for producing citric acid, for this strain was obtained from A. niger (ATCC) citric acid producing subsequently mounted analytical techniques: biomass dry weight substrate and DNS method citric acid by the method of pyrimidine. Then optimized the culture medium to remove excess salts (Fe2 +, Mn2 +, Zn2 +, Cu2 +). Later process was optimized in shake flasks of 500 ml nominal volume of 200 mL of culture medium at 200 rpm's being the best production. Finally, I simulate the process scaling to reactors 10 and 350,000 liters. The simulation process: fermentation and purification was 8 days, obtaining 27 tons of citric acid with an investment of 1, 616,723.00 USD.
