Resumen de Lower expression of ER-?36 is associated with the development of endometrial hyperplasia in PCOS patients

Sheng-Li Lin, Bin-Bin Tu, Xiao-Guo Du, Li-Ying Yan, Jie Qiao

• Objective: To explore the expression change of ER-?36 in endometria of PCOS patients. Methods: Proliferative endometria were collected and divided into three groups: CE group (n=30), proliferative endometria from control women; PCOSE group (n=30), proliferative endometria from PCOS patients; HPCOSE group (n=15), hyperplastic endometria from PCOS patients. The cellular localization of ER-?36 and ER-?66 was analyzed by immunohistochemistry, and expression of ER-?36 and ER-?66 was determined by immunohisto-chemistry and Real-Time Quantitative PCR. The correlation between serum hormone concentration and expression of ER-?36 and ER-?66 was analyzed. Results: ER-?36 was localized on the cell surface, cytoplasm and nucleus of glandular epithelial cells of both CE and PCOSE groups, mainly on the cell surface, and ER-?66 was localized on the nucleus. The protein and mRNA expression of ER-?36 was decreased from CE, PCOSE to HPCOSE group, while expression of ER-?66 showed no obvious difference among groups. The expression ratio of ER-?36 to ER-?66 of CE, PCOSE and HPCOSE group showed a decreasing tendency. The expression of ER-?36 and its expressive ratio to ER-?66 was negatively correlated with serum concentration of LH, LH/FSH, testosterone and androstenedione. Conclusions: Lower expression of ER-?36 is associated with the development of endometrial hyperplasia in PCOS patients.