Specific igg and immune complex to parthenogenetic females and eggs of strongyloides venezuelensis in the serum samples from rats experimentally infected

• Autores: Ana Lúcia Ribeiro Gonçalves, Karine Canuto Loureiro de Araújo, Edson Fernando Carvalho Goulart, Marlene Tiduko Ueta, Julia Maria Costa Cruz
• Localización: The Biologist, ISSN-e 1994-9073, ISSN 1816-0719, Vol. 10, Nº. Extra 2 (julio-diciembre), 2012
• Idioma: inglés
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• Resumen

  • In order to detect levels of antibody and immune complex by ELISA in serum samples using extracts and anti-IgG of parthenogenetic females or eggs from Strongyloides venezuelensis, non immunosuppressed or immunosuppressed rats experimentally infected used. The microtitre plates were coated with alkaline parasite extract of parthenogenetic females or eggs from Strongyloides venezuelensis for antibody detection and with anti-IgG of parthenogenetic females or eggs for immune complex detection. The statistical analyses were analyzed using Two Way ANOVA, followed by the Bonferroni test. The criterion for statistical significance was set at p<0.05. For the parthenogenetic female extract, it was observed that specific IgG detection in serum samples was not good approaches for evaluating the kineticss of infection in groups. Immune complex detection rates were significant increases mainly at day 5 post infection (p.i) (p< 0.05) for non immunosuppressed rats and for immunosuppressed rats it was detected during entire kineticss of infection. For the eggs extract, specific IgG showed lower levels in both groups with distinct increase at day 5 p.i (p< 0.01) in non immunosuppressed rats and at day 21 p.i (p< 0.001) in immunosuppressed rats. Immune complex was detected during entire kinetics of infection in immunosuppressed animals. When used the parthenogenetic female the diagnosis was more effective than when used the egg for immune complex detection in serum samples mainly from non immunosuppressed rats experimentally infected with S. venezuelensis. Then, parthenogenetic female to be an alternative for strongyloidiasis diagnosis and immune complex detection may be a good approach for strongyloidiasis diagnosis in activity.