A New Strategy for the Discrimination of Mitochondrial DNA Haplogroups in Han Population

• Autores: Yi Yang, Ping Ping, Qiang He, Yiling Zhu, Xianghong Yang, Rong Lv, Jianghua Chen
• Localización: Journal of forensic sciences, ISSN-e 1556-4029, ISSN 0022-1198, Vol. 56, Nº. 3, 2011, págs. 586-590
• Idioma: inglés
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• Resumen

  • Keywords:forensic science;mtDNA haplogroup;single nucleotide polymorphisms;hypervariable segments-I;real-time polymerase chain reaction;polymerase chain reaction�restriction fragment length polymorphism Abstract: Mitochondrial DNA (mtDNA) haplogroup discrimination is interesting not only for phylogenetic and clinical but also for forensic studies. We discriminated the mtDNA haplogroups of 570 healthy unrelated Han people from Zhejiang Province, Southeast China, by comprehensive analysis mutations of the hypervariable segments-I sequence and diagnostic polymorphisms in mtDNA coding region using real-time polymerase chain reaction (RT-PCR), which was compared with the widely used PCR and restriction fragment length polymorphism (PCR-RFLP) method. The results showed that in superhaplogroup M, haplogroup D was the most common haplotype within this assay to 24.6%, and in the other superhaplotype N, haplogroup B and F were the most common groups. Samples re-identified by PCR-RFLP showed the consistent results that were got with RT-PCR. In conclusion, the RT-PCR strategy appears to be an accurate, reproducible, and sensitive technique for the discrimination of mtDNA haplogroups, especially for mass screenings quickly and economically.