In vitro production of porcine embryos. New insights into optimization of culture media with relevance to sex ratio purposes
- Autores: Eva Torner Bosch
- Directores de la Tesis: Sergi Bonet (dir. tes.), Maria Dolors Briz González (dir. tes.), Eva Bussalleu Muntada (dir. tes.)
- Lectura: En la Universitat de Girona ( España ) en 2014
- Idioma: inglés
- Tribunal Calificador de la Tesis: Mercè Durfort i Coll (presid.), Josep Santaló (secret.), Silvia Sancho (voc.)
- Materias:
- Enlaces
- Tesis en acceso abierto en: TDX
- Resumen
This thesis is about challenges that currently concern in vitro production (IVP), sex determination procedures and sex-related survival of in vitro-produced porcine embryos. Specifically, the current thesis develops a new suitable PCR-based procedure based on the amplification of repetitive sequences for determining the sex of porcine embryos at different developmental stages. Furthermore, this work establishes new culture conditions and provides new knowledge about physiology, development and sexual dimorphisms in survival rates and kinetics of the development of in vitro-produced porcine embryos under specific in vitro culture conditions. The presence of optimal concentrations of hyaluronic acid and specific energy substrates at the appropriate time of embryo development, as well as the timing of several events during the earliest stages of development, essentially the timing of the first embryonic cleavage, appear to be linked amongst them and are related to subsequent embryonic competence, influencing development in a sex related manner
