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    Fuensanta García-Orenes

    Alkaline soils are considered much less prone to developing water repellency induced by fire than acidic soils. Here we report on the persistence of water repellency present in calcareous soils immediately after wildfires in 10 burned... more
    Alkaline soils are considered much less prone to developing water repellency induced by fire than acidic soils. Here we report on the persistence of water repellency present in calcareous soils immediately after wildfires in 10 burned areas in SE Spain, its distribution in different aggregate size fractions (<2, 2–1, 1–0.5, 0.5–0.25 and <0.25 mm) and on results from aggregate stability tests.
    Previous studies have shown that long-term irrigation with wastewater can lead to the development of soil water repellency. Little is known about the longevity of this effect. Here we address this research gap by examining the effect of... more
    Previous studies have shown that long-term irrigation with wastewater can lead to the development of soil water repellency. Little is known about the longevity of this effect. Here we address this research gap by examining the effect of long-term (~ 20 years) use of low-quality wastewater for disposal purposes, followed by 6 years of ‘recovery’ with no irrigation, on the wettability of calcareous sandy soil (Xerofluvent) under a Populus alba tree stand used as a “green filter” in SE Spain. Water repellency (WR) and soil organic matter content (SOM) were determined for 120 air-dry samples from the plot and 80 control samples from adjacent and otherwise similar non-irrigated areas. To account for plot micro-topography 40 samples each were taken from ridges (R; 0–5 cm depth), furrows (F; 0–5 cm), and furrows at depth (FD; 5–10 cm). The controls included 40 samples each (0–5 cm depth) from unvegetated and unploughed soil, and from soil under the P. alba plantation.All control samples were non-repellent whereas at the irrigated plot, water repellency was present for 48, 95 and 93% of ridge, furrow and furrow-depth samples respectively. WR and SOM was strongly correlated within the whole sample population (R2 = 0.623**) and within two sample groups (R: R2 = 0.783**; FD: R2 = 0.424**), but weakly within F samples (R2 = 0.072 n.s.). The latter showed the highest frequency and persistence (WDPT) of WR, indicating that not only quantity of SOM is controlling WR. Exploratory kaolinite clay additions (0.5–8%) to samples substantially reduced WR even at the lowest concentration, indicating that this could be a promising amelioration treatment for the WR in the soils investigated here.We conclude that for the conditions studied here (i) long-term use with poor-quality wastewater for disposal had led to the development of soil WR, (ii) a 6-year period of ‘recovery’ (i.e. non-irrigation) was insufficient to eliminate the induced WR, and (iii) kaolinite addition could be a promising amelioration treatment for these sandy soils.► Long-term application of wastewater with poor-quality had led to the development of soil WR. ► A 6-year period of ‘recovery’ has been insufficient to eliminate the induced WR. ► Kaolinite addition appears to be effective as an amelioration treatment for these sandy soils.
    [This corrects the article DOI: 10.1371/journal.pone.0080522.].
    Flood irrigated citrus orchard abandonment in eastern Spain is due to socio-economic changes and the small size of the farms. By means of a cylinder infiltrometer and measurements of soil organic matter we conclude that land abandonment... more
    Flood irrigated citrus orchard abandonment in eastern Spain is due to socio-economic changes and the small size of the farms. By means of a cylinder infiltrometer and measurements of soil organic matter we conclude that land abandonment favours an increase in total carbon and infiltration rates. The recovery showed a crisis in the first year after abandonment, when the vegetation did not recover and the soil was affected by a surface crust. Although the abandoned orchards did not receive fertilizers or irrigation, the abandonment is positive for recovery of organic matter and infiltration rates.
    Shortage of water is one of the most important environmental problem in the Mediterranean areas that implicates the search for strategies for saving good quality water. The use of treated waste water for the irrigation of agricultural... more
    Shortage of water is one of the most important environmental problem in the Mediterranean areas that implicates the search for strategies for saving good quality water. The use of treated waste water for the irrigation of agricultural land can be a good solution for this problem because it reduces the utilization of fresh water and potentially could improve soil key parameters, but can modify physical-chemical and biological properties of the same. The aim of this work was to study the effect of long-term irrigation with treated waste water on microbial diversity, mainly arbuscular mycorrhizal fungi (AMF) of the soil and other properties related with the microbial community. The experiment was developed in an agricultural area with Citrus orchard, located in Alicante in the southeast Spain. Here, we tested whether the communities of AMF as well as soil microbial properties were affected by irrigation with water coming from sewage treatment plant during 40 years in a soil. To carry o...
    El presente trabajo muestra una primera aproximación experimental para el seguimiento del proceso de compostaje a gran escala de un lodo de depuradora, mediante el empleo de radiometría visible e infrarrojo cercano (VNIR).
    ABSTRACT We investigated the diversity of arbuscular mycorrhizal fungi (AMF) in roots of Prunus persica under two fertilization treatments (CF: consisted of application of chicken manure (1400 kg.ha-1), urea (140 kg.ha-1), complex... more
    ABSTRACT We investigated the diversity of arbuscular mycorrhizal fungi (AMF) in roots of Prunus persica under two fertilization treatments (CF: consisted of application of chicken manure (1400 kg.ha-1), urea (140 kg.ha-1), complex fertilizer 12-12-17/2 (280 kg.ha-1), and potassium sulfate (40 kg.ha-1) and IF: consisted of application of urea (140 kg.ha-1), complex fertilizer 12-12-17/2 (400 kg.ha-1) and potassium sulfate (70 kg.ha-1)) combined with integrated pest management (IM) or chemical pest management (CM), in a tropical agroecosystem in the north of Venezuela. Our goal was to ascertain how different fertilizers/pest management can modify the AMF diversity colonizing P. persica roots as an important step towards sustainable soil use and therefore protection of biodiversity. The AM fungal small-subunit (SSU) rRNA genes were subjected to PCR, cloning, sequencing and phylogenetic analyses. Twenty-one different phylotypes were identified, which were grouped in five families: Glomeraceae, Paraglomeraceae, Acaulosporaceae, Gigasporaceae and Archaeosporaceae. Sixteen of these sequence groups belonged to the genus Glomus, two to Paraglomus, one to Acaulospora, one to Scutellospora and one to Archaeospora. A different distribution of the AMF phylotypes as consequence of the difference between treatments was observed. Thus, the AMF communities of tree roots in the (IF+CM) treatment had the lowest diversity (H'=1.78) with the lowest total number of AMF sequence types (9). The trees from both (CF+IM) and (IF+IM) treatments had similar AMF diversity (H'?2.00); while the treatment (CF+CM) yielded the highest number of different AMF sequence types (17) and showed the highest diversity index (H'=2.69). In conclusion, the crop management including combination of organic and inorganic fertilization and chemical pest control appears to be the most suitable strategy with respect to reactivate the AMF diversity in the roots of this crop and thus, the agricultural and environmental sustainability in the agroecosystem.
    ABSTRACT Land abandonment throughout the twentieth century led to an intense landscape transformation in the mountain areas of the Iberian Peninsula. In some cases, and after 50 years of abandonment, agriculture returned with the... more
    ABSTRACT Land abandonment throughout the twentieth century led to an intense landscape transformation in the mountain areas of the Iberian Peninsula. In some cases, and after 50 years of abandonment, agriculture returned with the development of commercial farms and the intensification of the agrarian activities. In the Easter Iberian Peninsula, following the abandonment of olive groves, vineyards and cereals during the 50's, has been in the past two decades the expansion of intensive citrus production on sloping terrain. Geomorphological transects and simulated rainfall experiments have quantified the impact of traditional rainfed cultivation of the 50's, abandonment, and the intensification of farming on the processes and landforms of erosion on the northern slopes of the Serra Grossa, south of the province of Valencia. It was found that the citrus groves have the highest number of rills and gullies. These erosive morphologies were not in the traditional crop tillage, and when they formed in the abandoned fields were soon controlled by the growth of vegetation. The abandonment of the crops reduced the soil losses, but the intensification of the agriculture with the citrus production has accelerated the soil erosion rates that exceed by several orders of magnitude (x4) the soil erosion rates measured during the abandonment and multiply by 17 the quantified traditional rainfed agriculture soil losses. Tillage of traditional rainfed orchards reduced runoff, but increased the concentration of sediments of the surface wash.
    ABSTRACT Agricultural land management greatly affects soil properties. Microbial soil communities are the most sensitive and rapid indicators of perturbations in land use and soil enzyme activities are sensitive biological indicators of... more
    ABSTRACT Agricultural land management greatly affects soil properties. Microbial soil communities are the most sensitive and rapid indicators of perturbations in land use and soil enzyme activities are sensitive biological indicators of the effects of soil management practices. Citrus orchards frequently have degraded soils and this paper evaluates how land management in citrus orchards can improve soil quality. A field experiment was performed in an orchard of orange trees (Citrus Sinensis) in the Alcoleja Experimental Station (Eastern Spain) with clay-loam agricultural soils to assess the long-term effects of herbicides with inorganic fertilizers (H), intensive ploughing and inorganic fertilizers (P) and organic farming (O) on the soil microbial properties, and to study the relationship between them. Nine soil samples were taken from each agricultural management plot. In all the samples the basal soil respiration, soil microbial biomass carbon, water holding capacity, electrical conductivity, soil organic matter, total nitrogen, available phosphorus, available potassium, aggregate stability, cation exchange capacity, pH, texture, macronutrients (Na, Ca and Mg), micronutrients (Fe, Mn, Zn and Cu), calcium carbonate equivalent, calcium carbonate content of limestone and enzimatic activities (urease, dehydrogenase, β-glucosidase and acid phosphatase) were determined. The results showed a substantial level of differentiation in the microbial properties, which were highly associated with soil organic matter content. The management practices including herbicides and intensive ploughing had similar results on microbial soil properties. O management contributed to an increase in the soil biology quality, aggregate stability and organic matter content.
    ABSTRACT Plant species can influence fire intensity and severity causing different immediate and long-term responses on the soil microbial community. The main objective of this work was to determine the role of two representative... more
    ABSTRACT Plant species can influence fire intensity and severity causing different immediate and long-term responses on the soil microbial community. The main objective of this work was to determine the role of two representative Mediterranean plant species as soil organic matter sources, and to identify their influence on microbial response before and after heat exposure.A laboratory heating experiment (300 °C for 20 min) was performed using soil collected under Pinus hallepensis (PIN) and Quercus coccifera (KER). Dried plant material was added before heating for a total of six different treatments: non-heated control samples amended with the original plant material (PIN0 and KER0); PIN samples heated with pine (PINp) or kermes oak litter (PINk); KER samples heated with kermes oak (KERk) or pine litter (KERp). Heated soils were inoculated with the original fresh soil and different microbial parameters related to abundance, activity and possible changes in microbial community composition and chemical soil parameters that could be conditioning microbial response were measured for 28 days after inoculation.The effect of heating on the soil microbial parameters studied was influenced to a small extent by the plant species providing fuel, being evident in soil samples taken under pine influence. Nevertheless heating effect showed marked differences when plant species influence on soil origin was analyzed.In general, samples taken under pine appear to be more negatively affected by heating treatment than samples collected under kermes oak, highlighting the importance of vegetation as a fresh organic matter source in soil ecosystems before and after fire.
    Gypsiferous soils have a high agricultural value, but their utilization is limited by the presence of gypsum that can induce hardpan and vertical crusting. This paper reports on sulphate reduction in this soil type as a basis of a... more
    Gypsiferous soils have a high agricultural value, but their utilization is limited by the presence of gypsum that can induce hardpan and vertical crusting. This paper reports on sulphate reduction in this soil type as a basis of a bioremediation technology to remove the gypsum content of calcareous gypsiferous soils. Both the endogenous and bioaugmented sulphate-reducing potential of the soil was assessed in anaerobic batch tests. An endogenous population of sulphate-reducing bacteria was found to be present in the calcareous gypsiferous soil investigated, which could form the basis of both in situ and ex situ bioremediation schemes for this soil type.
    The capacity of different microbial groups to recolonise soil after a fire event will be decisive in determining the microbial community after the fire. Microbial recovery after a wildfire that occurred in Sierra la Grana (Alicante... more
    The capacity of different microbial groups to recolonise soil after a fire event will be decisive in determining the microbial community after the fire. Microbial recovery after a wildfire that occurred in Sierra la Grana (Alicante province, southeast Spain) was tracked for 32 months after the fire. Colony forming units (CFUs) of different microbial groups, microbial biomass, soil respiration, bacterial growth (leucine incorporation) and changes in the microbial community structure (phospholipid fatty acid (PLFA) analysis) were determined directly after the fire and four times during the recovery period. Direct effects were reflected by low values of most microbiological variables measured immediately after the fire. Microbial biomass increased during the first year after the fire but was below the unburned reference site 32 months after the fire. Bacterial activity and soil respiration showed the highest values immediately after the fire, but decreased to values similar to that of the unburned reference site or even lower (respiration) 32 months after the fire. Colony forming units of bacterial groups estimated by the plate count method peaked 8 months after the fire, but then decreased, showing values similar to the unburned reference site at the end of the study, with the exception of spore formers, which were 20 times higher than the reference site 32 months after the fire. Fungal CFUs were more sensitive to the fire and recovered more slowly than bacteria. Fungi recovering less rapidly than bacteria were also indicated by the PLFA pattern, with PLFAs indicative of fungi being less common after the fire. The recovery of microbial biomass and activity was mirrored by the initially very high levels of dissolved organic carbon being consumed and decreasing within 8 months after the fire. The wildfire event had thus resulted in a decrease in microbial biomass, with a more bacteria-dominated microbial community.
    In Eastern Spain, almond trees have been cultivated in terraced orchards for centuries, forming an integral part of the Mediterranean forest scene. In the last decades, orchards have been abandoned due to changes in society. This study... more
    In Eastern Spain, almond trees have been cultivated in terraced orchards for centuries, forming an integral part of the Mediterranean forest scene. In the last decades, orchards have been abandoned due to changes in society. This study investigates effects of changes in land use from forest to agricultural land and the posterior land abandonment on soil microbial community, and the influence of soil physico-chemical properties on the microbial community composition (assessed as abundances of phospholipids fatty acids, PLFA). For ...
    Información del artículo Evolución de las poblaciones de hongos y bacterias viables en suelos degradados enmendados con lodo de depuradora.
    Información del artículo Seguimiento del proceso de compostaje de un lodo en la EDAR de Aspe (ALicante).
    Freshwater availability and soil degradation are two of the most important environmental problems in the Mediterranean area acerbated by incorrect agricultural use of irrigation in which organic matter is not correctly managed, the use of... more
    Freshwater availability and soil degradation are two of the most important environmental problems in the Mediterranean area acerbated by incorrect agricultural use of irrigation in which organic matter is not correctly managed, the use of low quality water for irrigation, and the inefficiency of dose irrigation. For these reasons strategies for saving water and for the restoration of the mean properties of soil are necessary. The use of treated waste water for the irrigation of agricultural land could be a good solution to these problems, as it reduces the utilization of fresh water and could potentially improve key soil properties. In this work we have been studying, for more than three years, the effects on soil properties of different doses of irrigation with waste water. Here we show the results on aggregate stability. The study is located in an agricultural area at Biar (Alicante, SE of Spain), with a crop of grape (Vitis labrusca). Three types of waters are being used in the irrigation of the soil: fresh water (control) (TC), and treated waste water from secondary (T2) and tertiary treatment (T3). Three different doses of irrigation have been applied to fit the efficiency of the irrigation to the crop and soil type: D10 (10 L m-2 every week during 17 months), D50 (50 L m-2 every fifteen days during 14 moths) and D30 (30 L m-2 every week during 6 months up to present day). The results showed a clear decrease of aggregate stability during the period we used the second dose (D50) independent of the type of water used. That dose of irrigation and frequency produced strong wetting and drying cycles (WD) in the soil, and this is suspected to be the main factor responsible for the results. When we changed the dose of irrigation to D30, reducing the quantity per event and increasing the frequency, the soil aggregate stability started to improve. This dose avoids strong drying periods between irrigation events and the aggregate stability is confirmed to be slowly increasing. A study in the medium or long-term is necessary to continue to ascertain the impact on soil of the irrigation and to assess the feasibility of using these waters in this type of soil. Aknowledgements: This research was supported by the Water Reuse project (Reference STREP- FP6-2003-INCO-Russia+NIS-1. PL 516731). A. Morugán acknowledge the grants from 'Caja Mediterraneo'. The authors also acknowledge the "Biar waste water treatment station", 'Entidad pública de saneamiento de aguas residuales de la Comunidad Valenciana' and "Proaguas Costablanca" for the collaboration and to Frances Young for improving the English.
    One of the consequences of long-term irrigation with waste water can be the development of soil water repellency (WR). Its emergence can affect soil-water balance, irrigation efficiency and crop yield. Water repellency development has... more
    One of the consequences of long-term irrigation with waste water can be the development of soil water repellency (WR). Its emergence can affect soil-water balance, irrigation efficiency and crop yield. Water repellency development has been suggested to be controlled by parameters such as organic matter quantity and type present in the waste water, soil properties (particularly the texture), and the overall time period of irrigation. Here we examine the effect of long-term (~20 years) irrigation with low quality waste-water on soil wettability under a Populus alba tree stand used as a "green filter". The plot exhibited considerable micro-topography (ridges and furrows) and consisted of sandy calcareous soil (Xerofluvent). Water repellency and organic carbon content (OC) were studied in 160 samples taken from the plot and from an adjacent area used as control (no irrigated). From the control area 40 samples were taken from the first 5 cm of mineral soil (C samples). From the...
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