Choice of the adequate quantification method for recombinant human GM-CSF produced in different host systems

• Mariela Bollati Fogolín ^[1] ; Marcos Oggero Eberhardt ^[1] ; Ricardo Kratje ^[1] ; Marina Etcheverrigaray ^[1]
  1. [1] Universidad Nacional del Litoral

     Universidad Nacional del Litoral

     Argentina

     
• Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 5, Nº. 3, 2002, págs. 15-16
• Idioma: inglés
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• Resumen

  • Three enzyme-linked-immunosorbent assays (ELISA) were developed and compared with a bioassay to quantify the recombinant human granulocyte-macrophage colony stimulating factor (rhGM-CSF). These assays were suitable to quantify the non-glycosylated rhGM-CSF present in mixtures with variable protein content, and therefore useful for determining concentrations of the cytokine in production processes. Among these assays, the competitive ELISA, developed with a MAb that recognises an epitope not involved in glycosylation, was the only one suitable to quantify the glycosylated form of rhGM-CSF produced in mammalian cell cultures.