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Recombinant expression of marine shrimp lysozyme in Escherichia coli

    1. [1] Universidad de Sonora

      Universidad de Sonora

      México

    2. [2] Centro de Investigación en Alimentación y Desarrollo

      Centro de Investigación en Alimentación y Desarrollo

      México

  • Localización: Electronic Journal of Biotechnology, ISSN-e 0717-3458, Vol. 7, Nº. 3, 2004, págs. 12-13
  • Idioma: inglés
  • Enlaces
  • Resumen
    • Shrimp Lysozyme (Lyz) is a key component of the antibacterial response as part of the innate defense in Crustacea; however, it has not been possible to purify this protein because of the very low amount present in the shrimp blood cells (hemocytes). In an effort to produce enough protein to study its function and biochemical properties we have overexpressed Lysozyme from marine shrimp (Penaeus vannamei) in E. coli. A bacterial protein expression system based on the T7 polymerase promoter was used. Although Lyz was produced as insoluble protein in inclusion bodies, its refolding led to an active protein with a yield of ~10%. Details of the protein recombinant expression techniques applied to this shrimp protein are presented

Los metadatos del artículo han sido obtenidos de SciELO Chile

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