Quantitative evaluation of bias in barcode markers derivedfrom complex samples

• Autores: Marta Pawluczyk, Julia Rosl Weiss, Matthew G. Links, E. Aranguren, Mark Wilkinson
• Localización: Actas del III Workshop en Investigación Agroalimentaria. WIA. 3.1 / Francisco Artés Hernández (dir. congr.), Marcos Egea Gutíerrez-Cortines (dir. congr.), Alfredo Palop Gómez (dir. congr.), Sebastián Bañón Arias (dir. congr.), Pablo Bielza Lino (dir. congr.), 2014, ISBN 978-84-697-1358-7, págs. 78-81
• Idioma: inglés
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• Resumen

  • PCR products have become a major commodity used to identify organisms based on polymorphism at the DNA level. One problem arising is that unbiased identification of organisms takes as working hypothesis that when DNA is extracted from a sample, a positive signal will be obtained if universal primers are used and DNA quality is suitable for PCR. As this assumption is not always correct we used a system where large differences in PCR success have been described to identify where biases appear and maybe identify solutions. Plants can be identified with at least seven independent plastid‐located loci. These differ in their degree of PCR success and how informative they are in terms of taxonomically useful sequence polymorphisms. Here we used six common plastid loci spanning 48 plant species and performed a quantitative analysis of bias at each step of the identification process. As expected we found important differences in PCR efficiency within a single species, depending on the ...