Resumen de Characterization of grape cultivars through estp

Priscila Sawasaki Iamagutti, Marcelo F. Moura

• Grapevine cultivars developed by Instituto Agronômico de Campinas known as ?IAC 138-22 Máximo?, ?IAC Rainha? and ?IAC Madalena? were characterized to find a distinct genetic profile in order to facilitate patenting of a new cultivar. The characterization was done using the Expressed Sequence Tag Polymorphism (ESTP) methodology. From thirteen primers derived from EST initially chosen, nine amplified DNA fragments in sufficient quantity for sequencing, and only three gave different alleles corresponding to nine single nucleotide polymorphisms (SNP). Although the SNP frequency of about 1.2 per locus, observed in this study was low, by analyzing the sequences it was possible to detect specific restriction sites for the differentiation of cultivars ?IAC 138-22 Máximo?, ?IAC Rainha? and ?IAC Madalena?.