An Inexpensive Device for Capillary Electrophoresis with Fluorescence Detection

• Jonathan E. Thompson ^[1] ; Khriesto Shurrush ^[1] ; Greg Anderson ^[2]
  1. [1] University of Nebraska at Kearney

     University of Nebraska at Kearney

     Estados Unidos

     
  2. [2] Department of Chemistry, Troy State University, United States
• Localización: Journal of chemical education, ISSN 0021-9584, Vol. 83, Nº 11, 2006, pág. 1677
• Idioma: inglés
• Texto completo no disponible (Saber más ...)
• Resumen

  • We describe an inexpensive device for performing capillary electrophoresis (CE) separations with fluorescence detection. As a demonstration of the device's utility we have determined the mass of riboflavin in a commercially available dietary supplement. The device allows for separation of riboflavin in ~100 s with a mass limit of detection of ~10 fmol. The device may be of particular interest to faculty at smaller chemistry departments that do not currently possess a CE instrument. While of limited utility, the device we describe could be used to introduce students to capillary electrophoresis despite the obstacle of limited funding.