- Universidad Nacional del Nordeste, Facultad de Odontología, Faculty Memberadd
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Trabajo presentado en el XXXIII Congreso de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Córdoba, del 14 al 17 de septiembre de 2010Peer reviewe
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Research Interests: Biology, Medicine, Cell separation, Biological Sciences, Animals, and 15 moreMale, Research article, Myosin, Peptides, Trypsin, tESTIS, Alkaline phosphatase, Solubility, Rats, Amino Acid Sequence, Biology Reproduction, Spermatic Cord, Seminiferous tubules, Molecular Sequence Data, and Medical and Health Sciences
Background: In the dental clinic impacted teeth are frequent findings, especially upper and lower third molars, leading to their exodontia. Among surgical techniques piezosurgery is advantageous for delicate structures in the oral cavity.... more
Background: In the dental clinic impacted teeth are frequent findings, especially upper and lower third molars, leading to their exodontia. Among surgical techniques piezosurgery is advantageous for delicate structures in the oral cavity. Extracted teeth, usually discarded, have been revalued as biological material, providing living tissues with possible applications in regenerative dentistry. The aim was to compare cross-section methods of upper included third molars by ultrasonic piezoelectric technique to obtain dental pulp, with diamond-coated tip (DT) against titanium nitride-coated tip (TN), according to the pulp tissue cell viability and the section surface characteristics.
Material and methods: Patients attending dental consultation were evaluated. Upper third molars (n= 24) were avulsed from 15 patients with exodontia indication, age 18-26 years old, who agreed to participate of the study. Third molars were cross-sectioned at amelocemental junction level with piezoelectric device using DT or TN inserts. Pulps were mechanic and enzymatically treated, and tissue viability determined by Trypan Blue test. Sectioned teeth were visualized using Scanning Electron Microscope (SEM). Ethical principles of biomedical research were respected; all patients gave their informed consent.
Results: Viability of pulp tissue was 84.71% not associated to sex (p= 0.141) nor to teeth position, upper right third molar or upper left third molar (p= 0.580). According to the insert used, pulp tissue viability was 85.21% with TN, similar to 84.00% with DT (p= 0.611). By SEM, cut performed by TN insert showed smooth and uniform surfaces, while DT insert surfaces were irregular, porous, with fissures.
Conclusions: Piezosurgery applied to cross-section upper third molars with both types of inserts showed differences in the cut surfaces but similar effectiveness regarding preservation of pulp tissue with high viability, thus, they could be allocated for further cellular developments. Key words:Impacted teeth, third molars, piezosurgery, regenerative dentistry.
Material and methods: Patients attending dental consultation were evaluated. Upper third molars (n= 24) were avulsed from 15 patients with exodontia indication, age 18-26 years old, who agreed to participate of the study. Third molars were cross-sectioned at amelocemental junction level with piezoelectric device using DT or TN inserts. Pulps were mechanic and enzymatically treated, and tissue viability determined by Trypan Blue test. Sectioned teeth were visualized using Scanning Electron Microscope (SEM). Ethical principles of biomedical research were respected; all patients gave their informed consent.
Results: Viability of pulp tissue was 84.71% not associated to sex (p= 0.141) nor to teeth position, upper right third molar or upper left third molar (p= 0.580). According to the insert used, pulp tissue viability was 85.21% with TN, similar to 84.00% with DT (p= 0.611). By SEM, cut performed by TN insert showed smooth and uniform surfaces, while DT insert surfaces were irregular, porous, with fissures.
Conclusions: Piezosurgery applied to cross-section upper third molars with both types of inserts showed differences in the cut surfaces but similar effectiveness regarding preservation of pulp tissue with high viability, thus, they could be allocated for further cellular developments. Key words:Impacted teeth, third molars, piezosurgery, regenerative dentistry.
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En 1970 Michael y colaboradores (Michael, Blau y Vernier 1970) mostraron que la cara luminal de los podocitos o celulas epiteliales del glomerulo renal, estaban recubiertas de una superficie que podia ser digerida con neuraminidasa a la... more
En 1970 Michael y colaboradores (Michael, Blau y Vernier 1970) mostraron que la cara luminal de los podocitos o celulas epiteliales del glomerulo renal, estaban recubiertas de una superficie que podia ser digerida con neuraminidasa a la cual denominaron “polianion epitelial” por su afinidad por colorantes cationicos. Posteriormente, otros autores (Kerjaschki, Sharkey y Farquhar 1984), aislaron una sialoproteina glomerular de unos 140 kDa, tingible por colorantes cationicos, digerible por neuraminidasa y capaz de fijarse a aglutinina de germen de trigo. Estos autores concluyeron que esta proteina era el equivalente bioquimico del polianion del epitelio glomerular, descrito por medios histoquimicos, y la denominaron podocalicina (PODXL) dada su localizacion en el glicocalix de los podocitos glomerulares. Trabajos posteriores demostraron que su carga negativa era debida a su alto contenido en sulfato y acido sialico (Dekan, Gabel y Farquhar 1991). Se ha considerado que la podocalicina o, para ser mas precisos, su carga superficial, es esencial para mantener la estructura de las celulas epiteliales (podocitos) de los glomerulos renales e impedir la oclusion de las rendijas epiteliales, tambien denominadas espacios urinarios (Charest y Roth 1985, Kerjaschki y col. 1984, Michael y col. 1970). La importancia patologica de la podocalicina se apoya en los hallazgos de la disminucion del contenido glomerular de acido sialico, distorsion de la estructura de los podocitos y obliteracion de las rendijas epiteliales en casos de nefrosis humana de “cambios minimos” o en nefrosis experimentales (Caulfield, Reid y Farquhar 1976, Seiler y col. 1977). CD34, podocalicina y endoglicano se agrupan como una familia de proteinas (familia CD34) en base a la conservacion de la organizacion de sus dominios asi como de su organizacion genomica (Doyonnas y col. 2001, Nielsen y col. 2002, Li y col. 2001).
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Research Interests: Chemistry, Medicine, Platelet, Platelet aggregation, Mice, and 15 morePlatelets, Animals, Megakaryocytes, PT, Green Fluorescent Protein, Platelet activation mechanism, Renal Function, Clinical Sciences, Thrombosis, Bleeding Time, Fusion Protein, Epithelial Cell Biology, Control Group, Blood platelets, and Hyperlipidemias
Research Interests: Cognitive Science, Magnetic Resonance Imaging, Biology, Cell Migration, Medicine, and 15 moreBrain development, Brain, Molecular and cellular biology, Humans, Kidney, Mice, Female, Animals, Phenotype, Cellular and Molecular Neuroscience, Carotid Artery, Knock out, Mr Imaging, Epithelial Cell Biology, and Neurosciences
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Research Interests: Medical Microbiology, Biology, Immunohistochemistry, Transmission Electron Microscopy, Medicine, and 15 moreBiological Sciences, Humans, Animals, Physical sciences, Green Fluorescent Protein, Matrix Metalloproteinases, Base Sequence, Fusion Protein, Plasma Membrane, Extracellular Space, Biochemistry and cell biology, Matrix Metalloproteinase, CHO cells, Protein Kinase C, and Cricetinae
Research Interests: Biology, Medicine, Multidisciplinary, Platelet aggregation, Mice, and 15 moreAnimals, Megakaryocytes, Blood Coagulation, Platelet activation mechanism, PLoS one, Gene Targeting, Carotid Artery, Hemorrhage, Bleeding Time, Life Span, Ferric Compounds, Chlorides, Carotid Arteries, Cell count, and Blood platelets
Podocalyxin (PODXL) is a type I membrane sialomucin, originally described in the epithelial cells (podocytes) of kidney glomeruli. PODXL is also found in extra-renal tissues and in certain aggressive tumors, but its precise... more
Podocalyxin (PODXL) is a type I membrane sialomucin, originally described in the epithelial cells (podocytes) of kidney glomeruli. PODXL is also found in extra-renal tissues and in certain aggressive tumors, but its precise pathophysiological role is unknown. Expression of PODXL in CHO cells enhances their adhesive, migratory and cell–cell interactive properties in a selectin and integrin-dependent manner. We aimed at defining the PODXL domains responsible for those cell responses. For this purpose we have analyzed the cell adhesion/migration responses to deletion mutants of human PODXL, and the correlation with the activities of Rac1 and Cdc42 GTPases. The results obtained indicate that integrity of the PODXL ectodomain is essential for enhancing cell adhesion but not migration, while the integrity of the cytoplasmic domain is required for both adhesion and migration. Deletion of the carboxy-terminal DTHL domain (PODXL-ΔDTHL) limited only cell adhesion. The activities of Rac1 and Cdc42 GTPases parallel the PODXL-induced variations in cell adhesion and migration. Moreover, silencing the rac1 gene virtually abolished the effect of PODXL in enhancing cell adhesion.
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Malignant melanoma represents the fastest growing public health risk of all cancer types worldwide. Several strategies and anti-cancer drugs have been used in an effort to improve treatments, but the development of resistance to... more
Malignant melanoma represents the fastest growing public health risk of all cancer types worldwide. Several strategies and anti-cancer drugs have been used in an effort to improve treatments, but the development of resistance to anti-neoplastic drugs remains the major cause of chemotherapy failure in melanomas. Previously, we showed that the sesquiterpene lactone, dehydroleucodine (DhL), promotes the accumulation of DNA damage markers, such as H2AX and 53BP1, in human tumor cells. Also DhL was shown to trigger either cell senescence or apoptosis in a concentration-dependent manner in HeLa and MCF7 cells. Here, we evaluated the effects of DhL on B16F0 mouse melanoma cells in vitro and in a pre-clinical melanoma model. DhL inhibited the proliferation of B16F0 cells by inducing senescence or apoptosis in a concentration-dependent manner. Also, DhL reduced the expression of the cell cycle proteins cyclin D1 and B1 and the inhibitor of apoptosis protein, survivin. In melanomas generated by subcutaneous injection of B16F0 cells into C57/BL6 mice, the treatment with 20 mg DhL /Kg/day in preventive, simultaneous and therapeutic protocols reduced tumor volumes by 70%, 60% and 50%, respectively. DhL treatments reduced the number of proliferating, while increasing the number of senescent and apoptotic tumor cells. To estimate the long-term effects of DhL, a mathematical model was applied to fit experimental data. Extrapolation beyond experimental time points revealed that DhL administration following preventive and therapeutic protocols is predicted to be more effective than simultaneous treatments with DhL in restricting tumor growth.
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Peritubular myoid cells contain abundant actin filaments and they are contractile. Previously, we have show that rat myoid cells contain an isotype of smooth muscle myosin which is not assembled in filaments. In order to determine whether... more
Peritubular myoid cells contain abundant actin filaments and they are contractile. Previously, we have show that rat myoid cells contain an isotype of smooth muscle myosin which is not assembled in filaments. In order to determine whether this myosin isotype is able to assemble in vitro, myosin of rat myoid cells was purified and it´s salt-dependent solubility was assessed in a buffer containing different concentrations of NaCl. 200 mg/ml of myosin in phosphate-buffered 350 mM NaCl were dialyzed for 12 h again 100-350 mM NaCl. The samples where centrifuged, the pellets and supernatants analyzed by 7.5% SDS-PAGE, and the fraction of assembled myosin quantified by comparison of staining intensity of the bands. The assay showed that 95% of the myosin in 100 and 150 mM NaCl was assembled in filaments, 50% in 200 mM NaCl, and 10% in 350 mM NaCl. These results indicates that myoid cell myosin is able to assemble in vitro in a similar manner to other smooth muscle myosin II isotypes.
During rat testis development, seminiferous tubules (ST) growth to achieve the adults diameter. Peritubular myoid cells (PMCs), that surround ST, express the cytoskeletal markers of true smooth muscle (α-isoactin and PMC-myosin). With the... more
During rat testis development, seminiferous tubules (ST) growth to achieve the adults diameter. Peritubular myoid cells (PMCs), that surround ST, express the cytoskeletal markers of true smooth muscle (α-isoactin and PMC-myosin). With the aim to know the patter of PMC differentiation, the expression of PMC-myosin isotypes and the morphology of PMCs during rat development were analysed. PMC myosin isotypes were purified and analyzed in 4% Urea-SDS-PAGE. To determinate the patter of PMC contraction, STs were stimulated to contraction with Endotelin 1 (E-1). PMC´s morphology (x,y axes and surface) was assayed with Scion Image I Program from AgNO3 stained STs. PMCs of 12 days old rats expressed 3 isotypes of PMC- myosin: 205 (A), 200 (B) and 195 (C) kDa respectively, where the relative percent of expression was: A 20; B 20; C 60. PMCs of 16-27 year old rats expressed: A 33; B 32, C 35. Meanwhile rats of 43-90 days old expressed: A 45; B 45, C 10. PMC´s x, y axes form 16 year old rats we...
Deshidroleucodina (DhL) es una lactona sesquiterpénica del grupo de los guainolidos que promueve la acumulación de marcadores de daño al DNA en células HeLa y desencadena el ingreso de las células al estado de senescencia o apoptosis... more
Deshidroleucodina (DhL) es una lactona sesquiterpénica del grupo de los guainolidos que promueve la acumulación de marcadores de daño al DNA en células HeLa y desencadena el ingreso de las células al estado de senescencia o apoptosis según la concentración de DhL utilizada. Nos preguntamos si DhL puede producir en la misma célula senescencia y luego apoptosis. Células HeLa se trataron con 20 μM DhL (concentración que genera senescencia) y posteriormente con 30 μM DhL (concentración que produce apoptosis). Se analizaron en la misma célula la presencia de signos de senescencia y apoptosis. Actividad de la enzima SA-beta-Gal con el substrato X-Gal (indica senescencia) y reacción de Anexina V-FITC (indica apoptosis). Los experimentos se repitieron 3 veces y los resultados, media ± SE, se compararon por análisis de la varianza. Las células incubadas con DMSO (vehículo) por 48 y 72 h, presentaron senescencia en el 20 ± 2 y 21 ± 3% respectivamente y apoptosis en el 4 ± 0,2 y 3 ± 0,5% respe...
In the mammalian testis, peritubular myoid cells (PM cells) surround seminiferous tubules (ST) and participate in their contraction. PM cells contain myofilaments (MFs) arranged in two independent layers, the outer and the inner ones with... more
In the mammalian testis, peritubular myoid cells (PM cells) surround seminiferous tubules (ST) and participate in their contraction. PM cells contain myofilaments (MFs) arranged in two independent layers, the outer and the inner ones with longitudinal and transverse MFs to the ST axis. Endothelin-1 induces PM cells contraction by binding to its receptors (ETRs) localized in fixed caveolae. Due to the arrangements of MFs in PM cells, we asked if the localization of caveolae and ETRs receptors follow the MFs orientation. For this purpose, ST from adult rat testes were fixed with 4% paraformaldehyde for immunofluorescence (IF) or with 5% glutaraldehyde for transmission electron microscopy (TEM). For IF, MFs were visualized with anti-alpha actin antibody (Ab), caveolae using anti-caveolin-1 Ab, and ETRs with ETAR and ETBR Abs. By confocal microscopy, caveolin-1 and ETRs appear in the outer and the inner plasma membranes of PM cells in rows following the orientation of MFs bundles in the...
Podocalyxin (PODXL) is a type I transmembrane glycoprotein initially described in the epithelial cells of the kidney glomeruli, postulated to exert antiadhesive properties; however, we have previously observed that heterologous expression... more
Podocalyxin (PODXL) is a type I transmembrane glycoprotein initially described in the epithelial cells of the kidney glomeruli, postulated to exert antiadhesive properties; however, we have previously observed that heterologous expression of recombinant PODXL enhanced the adherence of cells to immobilized ligands, spreading, migration, as well as the interaction with other cells. The enhancing effect of PODXL on cell adhesion and migration decreased significantly in deletion mutants of the cytosolic domain or the DTHL domain. Thus, the integrity of the cytosolic carboxylic domain of PODXL, included the terminal DTHL domain, is required for PODXL to exert its effect on cell adhesion and cell-cell interactions. In the podocytes of kidney glomeruli the DTHL domain of PODXL interacts with ezrin and NHERF to establish a link with the actin cytoskeleton, essential for the function of podocytes. To determine whether the recombinant PODXL expressed in CHO cells was also linked to the cytosk...
La podocalicina (PODXL) es una proteína perteneciente a la familia CD34, se expresa en una amplia variedad de tejidos y tumores. Debido a su presencia en éstos se le considera un marcador de progresión y malignidad y se sabe que la... more
La podocalicina (PODXL) es una proteína perteneciente a la familia CD34, se expresa en una amplia variedad de tejidos y tumores. Debido a su presencia en éstos se le considera un marcador de progresión y malignidad y se sabe que la sobreexpresión en sistemas heterólogos tiene capacidad de adhesión y migración. El objetivo de nuestro trabajo es estudiar las propiedades de adhesión y migración de las células que expresan PODXL. Para ello preparamos construcciones de PODXL y mutantes de deleción del ectodominio (Δ45, Δ146 y Δ365 aa) como proteínas de fusión con la proteína verde fluorescente y se transfectaron de forma estable en células de ovario de hámster chino. Nuestros resultados muestran que las células que expresan PODXL, poseen una capacidad de adhesión y migración mayor que las células que no la expresan. La deleción de los primeros 45 aminoácidos, poseen propiedades de adhesión y migración mayores que las células que expresan PODXL y en cambio las mutantes Δ146 y Δ365, no pro...
ABSTRACT Phagocytosis is an important mechanism against microorganisms and occurs in macrophages. Several receptors increase its phagocytic capacity, internalization and agent destruction. Leishmania spp prevents it, reproducing in... more
ABSTRACT Phagocytosis is an important mechanism against microorganisms and occurs in macrophages. Several receptors increase its phagocytic capacity, internalization and agent destruction. Leishmania spp prevents it, reproducing in macrophages. Macrophages J774 were infected with i) Leishmania sp and ii) Leishmania sp + Escherichia coli. Frotis at different time were carried out and stained with Giemsa. The percentage of infected macrophages and Leishmania/phagocit quantity was recorded under optical microscopy. An increase in infected macrophages was observed in the coinfected group after 2 hours. However, it showed no difference after 4 or 20 hours. The number of vacuoles/phagocit showed a high increase in the same group after 20 hours. In conclusion, E. coli coinfection increases macrophage captation of Leishmania at an early stage, while an increase in number of parasite occur very late. Other intracellular mechanisms involved with increased coinfection efficiency with E. coli as well as its medical perspectives are yet to be known
Vascular smooth muscle cell (VSMC) proliferation plays an important role in the development of atherosclerosis and in the vascular changes seen in hypertension. Dehydroleucodine (DhL) is a sesquiterpene lactone that inhibits cell... more
Vascular smooth muscle cell (VSMC) proliferation plays an important role in the development of atherosclerosis and in the vascular changes seen in hypertension. Dehydroleucodine (DhL) is a sesquiterpene lactone that inhibits cell proliferation in plant cells. In this paper, we study the effect of DhL in the proliferation of VSMCs stimulated with 10% fetal bovine serum (FBS). Very low concentrations of DhL (2-6 microM) inhibited VSMC proliferation and induced cell accumulation in G2. DhL did not affect the dynamics of 3H-thymidine incorporation, and did not modify either the activity of DNA polymerase or the incorporation of deoxyribonucleotides in an in vitro assay. Moreover, DhL did not induce apoptosis in VSMCs. These results indicate that DhL, in very low concentration, induces a transient arrest of VSMCs in G2. Our data show that VSMCs are especially sensitive to DhL effect, suggesting that DhL could be potentially useful to prevent the vascular pathological changes seen in hypertension and other vascular diseases.
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This manuscript presents findings from the first dichotomous data pooling analysis on clinical trials (CT) regarding the effectiveness of binding potassium. The results emanated from pairwise and network meta-analyses aiming evaluation of... more
This manuscript presents findings from the first dichotomous data pooling analysis on clinical trials (CT) regarding the effectiveness of binding potassium. The results emanated from pairwise and network meta-analyses aiming evaluation of response to commercial potassium-binding polymers, that is, to achieve and maintain normal serum potassium (n = 1,722), and the association between this response and an optimal dosing of renin-angiotensin-aldosterone system inhibitors (RAASi) needing individuals affected by heart failure (HF) or resistant hypertension, who may be consuming other hyperkalemia-inducing drugs (HKID) (e.g., β-blockers, heparin, etc.), and frequently are affected by chronic kidney disease (CKD) (n = 1,044): According to the surface under the cumulative ranking area (SUCRA), sodium zirconium cyclosilicate (SZC) (SUCRA >0.78), patiromer (SUCRA >0.58) and sodium polystyrene sulfonate (SPS) (SUCRA <0.39) were different concerning their capacity to achieve normokalemia (serum potassium level (sK+) 3.5–5.0 mEq/L) or acceptable kalemia (sK+ ≤ 5.1 mEq/L) in individuals with hyperkalemia (sK+ >5.1 mEq/L), and, when normokalemia is achieved, patiromer 16.8–25.2 g/day (SUCRA = 0.94) and patiromer 8.4–16.8 g/day (SUCRA = 0.41) can allow to increase the dose of spironolactone up to 50 mg/day in subjects affected by heart failure (HF) or with resistant hypertension needing treatment with other RAASi. The potential of zirconium cyclosilicate should be explored further, as no data exists to assess properly its capacity to optimize dosing of RAASi, contrarily as it occurs with patiromer. More research is also necessary to discern between benefits of binding potassium among all type of hyperkalemic patients, for example, patients with DM who may need treatment for proteinuria, patients with early hypertension, etc.
Systematic Review Registration: https://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42020185614, CRD42020185558, CRD42020191430.
Systematic Review Registration: https://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42020185614, CRD42020185558, CRD42020191430.
Galectin-3 is a lectine that binds beta-galactosides. It is involved in cardiac remodeling and fibrosis through the activation of macrophages and fibroblasts. ST2 is secreted by myocardial cells due to cardiac overload. These two... more
Galectin-3 is a lectine that binds beta-galactosides. It is involved in cardiac remodeling and fibrosis through the activation of macrophages and fibroblasts. ST2 is secreted by myocardial cells due to cardiac overload. These two biomarkers have been traditionally studied in the field of heart failure to guide medical therapy and detect the progression of the disease. Nevertheless, there are novel evidences that connect galectin-3 and ST2 with coronary heart disease, and, specially, with atrial fibrillation. The aim of this article is to concisely review the diagnostic and prognostic role of galectin-3 and ST2 in different cardiac diseases.
This systematic review and meta-analysis aim to provide scientific evidence regarding the effects of training on respiratory muscle training's impact with the PowerBreath ®. A systematic analysis based on the PRISMA guides and a conducted... more
This systematic review and meta-analysis aim to provide scientific evidence regarding the effects of training on respiratory muscle training's impact with the PowerBreath ®. A systematic analysis based on the PRISMA guides and a conducted research structured around the bases of Web of Science, Scopus, Medline/PubMed, SciELO y Cochrane Library Plus. Six articles published before January 2021 were included. The documentation and quantification of heterogeneity in every metaanalysis were directed through Cochran's Q test and the statistic I 2 ; additionally, a biased publication analysis was made using funnel plots, whose asymmetry was quantified Egger's regression. The methodological quality was assessed through McMaster's. PowerBreath ® administering a ≥ 15% resistive load of the maximum inspiratory pressure (PIM) achieves significant improvements (54%) in said pressure within 4 weeks of commencing the inspiratory muscle training. The maximal volume of oxygen (VO2max) considerable enhancements was achieved from the 6 weeks associated with the maximum inspiratory pressure ≥ 21.5% post inspiratory muscle training onwards. Conversely, a significant blood lactate concentration decrement occurred from the 4th week of inspiratory muscle training, after a maximum inspiratory pressure ≥ 6.8% increment. PowerBreath ® is a useful device to stimulate sport performance and increase pulmonary function.
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Introduction: The possibility of performing intense workouts without falling into states of chronic fatigue stimulates the use of devices that improve muscular and hormonal functionality in athletes. The Elevation Training Mask (Training... more
Introduction: The possibility of performing intense workouts without falling into states of chronic fatigue stimulates the use of devices that improve muscular and hormonal functionality in athletes. The Elevation Training Mask (Training Mask LLC) (ETM) allows the application of hypoxia during exercise. The ETM is integrated into training routines increasing the physical stimulus to improve performance.
Objective: We evaluated the impact of ETM on Workouts of the Day (WODs), muscular and hormonal behavior in Crossfit athletes.
Material and method: Prospective cohort study. During 12 weeks 20 Crossfit athletes trained 60 minutes 3 days a week were randomly divided into 2 groups, control group (CG) (n=10) and ETM group (EG) (n=10) applying an additional progressive simulated altitude between 914 and 2743 meters. WODs (press, squat, deadlift, total CF and grace), macular markers: lactate dehydrogenase (LDH); creatine kinase (CK); myoglobin (Mb) and hormones: testosterone (T); cortisol (C), were evaluated at 2 time points of the study: day 1 (T1) and day 84 (T2).
Results: All WODs and parameters LDH, CK, Mb, T and C showed no significant difference (p>0.05) in the time group interaction.
In EG, a substantially lower percentage change (Δ) between T1 and T2 was observed in Mb (-16.01 25.82), CK (6.16 26.05) and C (-0.18 4.01%) than in CG (Mb: -094 4.39; CK: 17.98 27.19; C: 4.56 3.44). The Δ T1-T2 in the WODs were similar.
Conclusion: After 12 weeks of training under simulated hypoxia conditions with ETM there are no improvements in athletic performance assessed by WODs. However, the greater tendency to decrease Mb, CK and C,
Objective: We evaluated the impact of ETM on Workouts of the Day (WODs), muscular and hormonal behavior in Crossfit athletes.
Material and method: Prospective cohort study. During 12 weeks 20 Crossfit athletes trained 60 minutes 3 days a week were randomly divided into 2 groups, control group (CG) (n=10) and ETM group (EG) (n=10) applying an additional progressive simulated altitude between 914 and 2743 meters. WODs (press, squat, deadlift, total CF and grace), macular markers: lactate dehydrogenase (LDH); creatine kinase (CK); myoglobin (Mb) and hormones: testosterone (T); cortisol (C), were evaluated at 2 time points of the study: day 1 (T1) and day 84 (T2).
Results: All WODs and parameters LDH, CK, Mb, T and C showed no significant difference (p>0.05) in the time group interaction.
In EG, a substantially lower percentage change (Δ) between T1 and T2 was observed in Mb (-16.01 25.82), CK (6.16 26.05) and C (-0.18 4.01%) than in CG (Mb: -094 4.39; CK: 17.98 27.19; C: 4.56 3.44). The Δ T1-T2 in the WODs were similar.
Conclusion: After 12 weeks of training under simulated hypoxia conditions with ETM there are no improvements in athletic performance assessed by WODs. However, the greater tendency to decrease Mb, CK and C,
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Background: In the dental clinic impacted teeth are frequent findings, especially upper and lower third molars, leading to their exodontia. Among surgical techniques piezosurgery is advantageous for delicate structures in the oral cavity.... more
Background: In the dental clinic impacted teeth are frequent findings, especially upper and lower third molars, leading to their exodontia. Among surgical techniques piezosurgery is advantageous for delicate structures in the oral cavity. Extracted teeth, usually discarded, have been revalued as biological material, providing living tissues with possible applications in regenerative dentistry. The aim was to compare cross-section methods of upper included third molars by ultrasonic piezoelectric technique to obtain dental pulp, with diamond-coated tip (DT) against titanium nitride-coated tip (TN), according to the pulp tissue cell viability and the section surface characteristics. Material and Methods: Patients attending dental consultation were evaluated. Upper third molars (n= 24) were avulsed from 15 patients with exodontia indication, age 18-26 years old, who agreed to participate of the study. Third molars were cross-sectioned at amelocemental junction level with piezoelectric device using DT or TN inserts. Pulps were mechanic and enzymatically treated, and tissue viability determined by Trypan Blue test. Sectioned teeth were visualized using Scanning Electron Microscope (SEM). Ethical principles of biomedical research were respected; all patients gave their informed consent. Results: Viability of pulp tissue was 84.71% not associated to sex (p= 0.141) nor to teeth position, upper right third molar or upper left third molar (p= 0.580). According to the insert used, pulp tissue viability was 85.21% with TN, similar to 84.00% with DT (p= 0.611). By SEM, cut performed by TN insert showed smooth and uniform surfaces, while DT insert surfaces were irregular, porous, with fissures. Conclusions: Piezosurgery applied to cross-section upper third molars with both types of inserts showed differences in the cut surfaces but similar effectiveness regarding preservation of pulp tissue with high viability, thus, they could be allocated for further cellular developments.
Mesenchymal stem cells (MSCs) are promising candidates for bone regeneration therapies due to their plasticity and easiness of sourcing. MSC-based treatments are generally considered a safe procedure, however, the long-term results... more
Mesenchymal stem cells (MSCs) are promising candidates for bone regeneration therapies due to their plasticity and easiness of sourcing. MSC-based treatments are generally considered a safe procedure, however, the long-term results obtained up to now are far from satisfactory. The main causes of these therapeutic limitations are inefficient homing, engraftment, and osteogenic differentiation. Many studies have proposed modifications to improve MSC engraftment and osteogenic differentiation of the transplanted cells. Several strategies are aimed to improve cell resistance to the hostile microenvironment found in the recipient tissue and increase cell survival after transplantation. These strategies could range from a simple modification of the culture conditions, known as cell-preconditioning, to the genetic modification of the cells to avoid cellular senescence. Many efforts have also been done in order to enhance the osteogenic potential of the transplanted cells and induce bone formation, mainly
by the use of bioactive or biomimetic scaffolds, although alternative approaches will also be discussed. This review aims to summarize several of the most recent approaches, providing an up-to-date view of the main developments in MSC based regenerative techniques.
by the use of bioactive or biomimetic scaffolds, although alternative approaches will also be discussed. This review aims to summarize several of the most recent approaches, providing an up-to-date view of the main developments in MSC based regenerative techniques.
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Several lesions or pathologies alter the symmetry, anatomical morphology and facial physiology of the patient affecting their quality of life; therefore they need to be adequately repaired. Customized prosthetic devices allow proper... more
Several lesions or pathologies alter the symmetry, anatomical morphology and facial physiology of the patient
affecting their quality of life; therefore they need to be adequately repaired.
Customized prosthetic devices allow proper correction of these alterations through surgical interventions. Thus it is
important to develop protocols aimed to design the prosthesis considering the anatomical parameters of the patient, its
placement and the comprehensive approach to each case.
Tissue engineering proposes new strategies for regeneration or replacement of tissues or organs, restoring the
shape and function to the affected area. Many therapeutic approaches are based on stem cells since they could make
the difference in promoting tissue acceleration and regeneration in clinical practice.
With the prospect of being implemented in the field of oral-maxillofacial reconstruction, these developments could
have positive effects on patient´s care, impacting on population health.
affecting their quality of life; therefore they need to be adequately repaired.
Customized prosthetic devices allow proper correction of these alterations through surgical interventions. Thus it is
important to develop protocols aimed to design the prosthesis considering the anatomical parameters of the patient, its
placement and the comprehensive approach to each case.
Tissue engineering proposes new strategies for regeneration or replacement of tissues or organs, restoring the
shape and function to the affected area. Many therapeutic approaches are based on stem cells since they could make
the difference in promoting tissue acceleration and regeneration in clinical practice.
With the prospect of being implemented in the field of oral-maxillofacial reconstruction, these developments could
have positive effects on patient´s care, impacting on population health.
Research Interests:
Research Interests: Multidisciplinary, Platelet aggregation, Mice, Animals, Megakaryocytes, and 14 moreShear Stress, Blood Coagulation, Platelet activation mechanism, PLoS one, Size Distribution, Thrombosis, Gene Targeting, Somatic Cell Count, Carotid Artery, Hemorrhage, Bleeding Time, Life Span, Ferric Compounds, and Chlorides
Research Interests: Medical Microbiology, Immunohistochemistry, Transmission Electron Microscopy, Biological Sciences, Humans, and 10 moreAnimals, Physical sciences, Green Fluorescent Protein, Matrix Metalloproteinases, Base Sequence, Fusion Protein, Plasma Membrane, Extracellular Space, Biochemistry and cell biology, and Matrix Metalloproteinase
Research Interests: Mass Spectrometry, Molecular Mechanics, Cytoskeleton, Platelet, Stroke, and 15 moreHumans, Pyruvate Kinase, Platelets, Female, Male, TRAP, Clinical Sciences, Middle Aged, Electrophoresis, Adult, Thrombosis, Two-Dimensional Gel Electrophoresis, Electroforesis, Blood Proteins, and Case Control Studies
Research Interests:
Research Interests:
Research Interests:
The restricted deletion of the podocalyxin gene in megakaryocytes perturbs the control of hemostasia. Podocalyxin (Podxl) is an adhesive sialoprotein of the CD34 family, expressed in platelets and exposed at the membrane surface upon... more
The restricted deletion of the podocalyxin gene in megakaryocytes perturbs the control of hemostasia.
Podocalyxin (Podxl) is an adhesive sialoprotein of the CD34 family,
expressed in platelets and exposed at the membrane surface upon stimulation
by physiological agonists, suggesting a regulatory role in
the platelet function(s). This work aimed at investigating the role of
Podxl in platelet function and hemostasia.
To elucidate the role of Podxl in platelets we used the Cre-LoxP gene
targeting methodology to generate mice with a restricted ablation of
the podxl gene in megakaryocytes. Mice with Podxl-null megakaryocytes
did not produce any apparent phenotypical changes, showing a
normal life span and fertility, and normal blood cells count. However,
the bleeding time was retarded and the aggregation of platelets
in response to physiological agonists was diminishsed in Podxl-null
platelets. The number, size-distribution and polyploidy were similar
in nul-Podxl megakaryocytes to the floxed controls. Podxl-null platelets
exhibited normal surface receptors content and normal stimulation
by agonists. However, the ferric chloride-induced occlusion of
the carotid artery was significantly retarded in the platelet Podxl-null
mice. Moreover, perfusion of uncoagulated blood from Podxl-null
mice in parallel flow chamber showed reduced platelet adhesion and
formation of aggregates.
To conclude, megakaryocyte/platelets Podxl is involved in the regulation
of hemostasis, likely acting as a platelet co-stimulator due to its
proadhesive properties.
Podocalyxin (Podxl) is an adhesive sialoprotein of the CD34 family,
expressed in platelets and exposed at the membrane surface upon stimulation
by physiological agonists, suggesting a regulatory role in
the platelet function(s). This work aimed at investigating the role of
Podxl in platelet function and hemostasia.
To elucidate the role of Podxl in platelets we used the Cre-LoxP gene
targeting methodology to generate mice with a restricted ablation of
the podxl gene in megakaryocytes. Mice with Podxl-null megakaryocytes
did not produce any apparent phenotypical changes, showing a
normal life span and fertility, and normal blood cells count. However,
the bleeding time was retarded and the aggregation of platelets
in response to physiological agonists was diminishsed in Podxl-null
platelets. The number, size-distribution and polyploidy were similar
in nul-Podxl megakaryocytes to the floxed controls. Podxl-null platelets
exhibited normal surface receptors content and normal stimulation
by agonists. However, the ferric chloride-induced occlusion of
the carotid artery was significantly retarded in the platelet Podxl-null
mice. Moreover, perfusion of uncoagulated blood from Podxl-null
mice in parallel flow chamber showed reduced platelet adhesion and
formation of aggregates.
To conclude, megakaryocyte/platelets Podxl is involved in the regulation
of hemostasis, likely acting as a platelet co-stimulator due to its
proadhesive properties.