INTRODUCTION
In the Cerrado biome, about 38 species (commonly known as "murici") have been registered in the Byrsonima. They have economic potential due to their edible fruits, cork production, recuperation of degraded areas purposes, medicinal properties as well as ornamental to be used in gardens and parks. In addition, Byrsonima plants usually produce nectar, oils, and are frequently visited by bees for pollination. Byrsonima basiloba Juss. (Malpighiaceae) is a shrub of rapid growth that produces one of the largest fruits of the genus. Hence, this species has been targeted for domestication and cultivation (Almeida et al. 1998), and in vitro propagation protocols for murici have been carried out by Silva et al. (2012) and Martendal et al. (2013).
Concerning the structural features of Byrsonima genus, Barros (1992) and Barroso et al. (1999) investigated the morpho-anatomy of fruit and seeds from several species. However, post-seminal anatomical studies of seedlings and young plants are rare of this genus in the literature. This kind of investigation is highly significant as the characterization of seedlings and young plants can be used for field identification and establishing parameters to assess the quality of micropropagated plants. The knowledge on this topic is crucial because it has been determined that in vitro conditions can generate anatomical changes that may significantly reduce the viability of the plants.
The morphology of initial phases of the post-seminal development of B. basiloba and other species were described by Silveira et al. (2013a, 2013b). Thus, the present work is a study of B. basiloba seed anatomical structure and two- month-old seedlings. At this age, the stem and root system has expanded and can be differentiated from one another.
MATERIAL AND METHODS
Mature fruits from five mother plants of Byrsonima basiloba were collected in Brasilia-DF in the following coordinates: S 15° 28' W 47° 30', S 15°44' W 47°52', S 15°37' W 47° 44'. Voucher specimens were deposited in the UB Herbarium (University of Brasilia) under registration numbers UB 10623, UB 11690, UB 11692, UB 11694, and UB 11695. The fruit was depulped under tap water, and for removal of the pulp remnants, it was put in the pathways of small domestic ants that removed all the remaining pulp within a few days. The morphological and anatomical study of the endocarp and the seeds was done by analyzing 15 endocarps and 15 seeds. The nomenclature used is based on the works of Barroso et al. (1999), Oliveira (2001), and Souza et al. (2009). The seedlings were obtained from seeds removed and then germinated in cups containing sand at room temperature, as described in Silveira et al. (2013b). Ten healthy seedlings of two months old were processed for anatomical analysis. This work considered seedlings as individuals formed from the germination of the seed until the complete expansion of the first two pairs of eophylls (Duke 1965, Souza et al. 2009).
For the anatomical study, dissociated material was obtained by treating the samples with Jeffrey´s solution. Additionally, the endocarps were cooked in an autoclave (1 atm of pressure at 120 oC for 12 h). Moreover, the plant material was fixed in FAA-50, dehydrated in an alcoholic series, and then embedded in either paraffin or Historesin, Leica. Transverse sections of 10 to 15 μm were obtained on a rotatory microtome, and the ribbons were expanded on microscopy slides, dewaxed, and then stained with fast green and safranin.
Hand sections were obtained on table microtome and tested for the presence of starch (Lugol), lipids (Sudan III), phenolic compound (Iron Chlorine), protein (Xylidine Ponceau), and polysaccharides (Schiff´s periodic acid) (Johansen 1940).
RESULTS
FRUIT AND SEED
Byrsonima basiloba fruit ripening started from bottom to top and took approximately four months to complete. The fruit was drupoid, depressed-globose with a three-layered pericarp containing (a) a thin and yellow exocarp; (b) 8 mm thick yellow mesocarp; and (c) an extremely sclerified endocarp. The endocarp (Fig. 1-3) had the same shape as the fruit, was brown, trilocular, woody, crested and measured about 13.40 mm (±1.24) wide, 10.15 mm (±1.16) long. Moreover, it had one seed per loculus, and therefore, was classified as a pyrene. Nonetheless, rarely all seeds were viable; most frequently, there was only one well-developed seed per fruit. The upper portion of the endocarp had a large suture with the shape of a three-pointed star, and at the bottom, there was a concavity where the pedicel was inserted. Anatomically, the endocarp was exclusively composed of irregularly arranged sclerified cells (Fig. 2) with large lumens. The dissociated material revealed the occurrence of sclereids, mostly brachio-sclereids and macrosclereids, as well as some fibers.
The seed shape was subglobose to angular, piriform, about 5 mm (±0.26) wide, and occupied the whole internal space of the endocarp loculus (Fig. 3). The tegument was membranaceous, smooth, and brown. B. basiloba embryo was ivory-colored, with long, foliaceous, circinate, anisophyllous cotyledons, which had disjointed apices (Fig. 4). The embryonary axis was short, straight, and occupied a small part of the seed (Fig. 4-5).
The epicotyl was extremely short with an undifferentiated five-cell layered plumule whose cells had a dense cytoplasm and conspicuous nuclei (Fig. 6). The apical region of the hypocotyl-root axis had a rudimentary root cap, which covers the root promeristem (Fig. 7-9). The embryo protoderm was uniseriate with cells dividing anticlinally. The ground meristem, both in the cortical and pith regions, was composed of cells with an incipient vacuolization. The procambium was composed of elongated cells with conspicuous nuclei throughout the embryo. In general, meristematic cells of both shoot and root meristems presented large nuclei and dense cytoplasm with abundant granules, most likely protein bodies. Histochemical tests detected the presence of lipids and proteins in embryo cells.
The cotyledons had a uniseriate epidermis consisted of cuboidal cells with rounded corners (Fig. 10-11). Differentiating stomata were observed in the epidermis. In the middle region of the cotyledon, the mesophyll was eight to nine cells wide. The first subepidermal layer (on the adaxial side) was palisade, with cells dividing periclinally. The remaining cell layers were formed by compact isodiametric cells. Histochemical tests showed large amounts of lipids and proteins on both adaxial and abaxial faces as well as in the mesophyll cells. No starch was detected in the embryo.
The seed coat was composed of two parts as following: a) the outer layer was the testa, and, b) the inner layer was the tegmen (Fig. 12-13). Moreover, internally to the tegmen a residual endosperm up to four cells long could be identified close to the embryo apex (Fig. 4-5). Furthermore, lipid and protein bodies were observed in the endosperm cells, as well as the presence of polysaccharide in the cell walls. Starch was not detected in these cells.
SEEDLINGS AND YOUNG PLANTS
Byrsonima basiloba seeds were germinated in vitro to facilitate data collection and presented an epigeous germination (Fig. 14). One-two days after inoculation the seeds were fully imbibed, and the tegument started to break. Subsequently, the cotyledons began to unwind, and the radicle began to elongate. Besides, between 5-8 days after inoculation, the cotyledons turned greenish. Around the 20th day, the cotyledons were oblong, dark green, with decurrent opposite basis, symmetrical, and foliaceous. The pivoting root was thick and grew straight and at this stage, the lateral roots had already been developed (Fig. 15).
Around the 30th day of cultivation, when the first eophylls appeared, the expansion of the epicotyl became noticeable, and the first pair of eophylls was produced. After 45 days, the young plant showed an epicotyl with pale green young leaves (Fig. 14), with trichomes on both surfaces.
After two months, the plant was approximately 8 cm long, from which 5 cm composed the root system, and 3 cm the shoot with 2 to 4 pairs of eophylls (Fig. 14). The root system was axial with lateral roots (Fig. 15). The connection between the main root and the hypocotyl was oblique to the stem axis (Fig. 15). The basis of the hypocotyl was thickened, which corresponded to the initial phase of xylopodium differentiation (Fig. 15). The cotyledons were, morphologically opposite, oblong, fleshy-membranous, with visible midrib, glabrous, with the ventral face greener than the dorsal face. The eophylls showed intrapetiolar stipules and a cross opposite phyllotaxy, which was perpendicular to the cotyledons. The shoot internodes were covered by whitish trichomes, typical of the family, which are named Malpighiaceae trichome. The apical bud was densely hairy and visible before the completion elongation of the eophylls.
The main root (Fig. 16-22) presented a reduced root cap (average length 110 μm) and was made of a conical arrangement of cells in different stages of differentiation. The meristematic region was short in average 70 μm long. The promeristem was made of approximately three layers of cells, anticlinally elongated, and each layer presenting four to five cells (Fig. 16-18). The protoderm, as well as the ground meristem, and the procambium were clearly differentiated from the promeristem, which was composed of few cells (Fig. 16-18). Above the meristematic region, the cortical parenchyma and vascular cylinder were already differentiated. In the procambium, protoxylem vessels with helical thickening distant from the promeristem about two to three layers of cells (Fig. 19-22). The vascular system was tetrarch with one cell layered pericycle (Fig. 19-20).
Metaxylem vessels started maturing 2 cm above the root apex. The cortex was composed of seven to nine layers of cells with a uniseriate endodermis. In the outer cortex region, there was an exodermis whose cells were slightly smaller than the cortical parenchyma (Fig. 21). The epidermis was uniseriate and made of tabular cells with abundant trichomes (Fig. 22).
In mature regions of the root, the central portion of the vascular system presented cells with lignified walls. The vascular cambium was already differentiated and produced secondary xylem between the bundles of primary xylem (Fig. 23-24). At this stage, the cortex kept an intact endodermis, which was discontinued in the regions where lateral roots developed (Fig. 24). The epidermis had a high density of trichomes.
The root connection to the hypocotyl was oblique (Fig. 14), and had a thickened region due to the increase in the number of cell layers on one side of the hypocotyl cortex. Therefore, the vascular system was eccentric in this region (Fig. 27 and 28). In addition, at this region the conducting tissue already presented a secondary growth, although the pericycle and the pith, both composed of parenchyma cells, were clearly visible (Fig. 28). The periderm had just begun to differentiate that originated from outer cells layers of the cortex (Fig. 28). The cotyledonary traces and gaps could be seen in this region (Fig. 28-30).
Close to the cotyledonary node the vascular bundle became centralized (Fig. 29 and 30). Although the cotyledons were visually opposite (Fig. 14), anatomically, they were not inserted at the same level. The cotyledonary traces revealed that the insertion point of both cotyledons differed a few millimeters from each other (Fig. 29 and 30). The cotyledonary basis was adnate, which gradually separated from the stem (Fig. 29 and 30). The epidermis of the stem had abundant trichomes (Fig. 29-30).
The cotyledons and the eophylls (Fig. 31-35) presented a midrib and two parallel veins on the margins of the blade. In both cases, the vascular bundle of the midrib was organized in a semicircle with the phloem placed on the abaxial side of the leaf, which characterized collateral bundle (Fig. 31 and 34). Moreover, the mesophylls of cotyledons and eophylls were dorsiventral, with a single-layered palisade parenchyma cells and the spongy parenchyma on the abaxial side (Fig. 32-33). The leaf margins were bent down with mesophyll with three layers of cells (Fig. 33).
The epidermis was uniseriate and amphistomatic whose cells had thin and slightly sinuous walls (Fig. 32-34). The stomata were paracytic or anomocytic, at the same level of the epidermis, and with a higher density on the abaxial surface (Fig. 33 and 35). A thin lipidic cuticle covered the epidermis. Malpighiaceae trichomes covered both faces of eophyll epidermis, with a higher density on the abaxial face (Fig. 35). Idioblasts containing druses were seen in the veins and the mesophyll.
The shoot apex (Fig. 36-39) was flat with reduced diameter. The region of the promeristem was made of a tunica containing two layers of cells and a four-cell layered corpus, with large nuclei (Fig. 36-37). The cells of the procambium were cylindrical, with nuclei in the center of the cell, while the cells of the ground meristem presented a smaller proportion of nucleus/cytoplasm (Fig. 36-37). The protoderm was covered by long trichomes while the procambium cells remained undifferentiated. In the differentiating zone, pith cells presented large vacuoles with druses (Fig. 38 and 39). In this region, the procambium cells were partially differentiated with vessel elements with helical thickening (Fig. 39).
DISCUSSION
Normally, fruit and seeds present small phenotypic plasticity. Therefore, Byrsonima typically presents a drupe fruit with a single pyrene divided into three loci (Anderson 1977, Barroso et al. 1999). Nevertheless, the crested endocarp observed in B. basiloba with a shape of three-pointed star suture had not yet been described in other species of the genus. Therefore, this feature has a significant taxonomic impact for the identification of this species in the field.
The anatomical structure of B. basiloba endocarp confers, at the same time, antagonistic properties: rigidity, lightness, and insulation. The large lumens of the sclerified cells and the irregular distribution of the cells not only reduce the density of the tissue but also help to insulate the seed. Consistently, the endocarp of B. intermedia A. Juss. is also composed of many layers of sclereids, which in this case, were elongated in the longitudinal sense of the fruit (Souto & Oliveira 2005). These findings can be related to the ecological strategies of seed dispersal. For instance, the sclerified endocarp of these species may insulate and protect the seed against the frequent fires in Cerrado, and simultaneously, the high temperatures can lead to the fracture of the endocarp, and thereby, to the release of the seed.
According to Boesewinkel & Bouman (1984), the reduction of the seed coat had been systematically observed in seeds with woody, indehiscent pericarps. In such cases the protective role of the seed is carried out by the pyrene. In Cerrado, it is common that the plants present hard sclerified and water impermeable seed coats, which must be removed by mechanical fracture or fire to allow the seed to imbibe and germinate (Palhares 2004). Furthermore, B. basiloba seeds are not dormant. However, the hard endocarp may be a barrier for water absorption; hence, it must be broken to allow seed germination.
Malpighiaceae seeds usually do not present endosperm. Rather, they have a thin brownish coat and foliaceous cotyledons (Anderson 1990, Barroso et al. 1999). This exalbuminous aspect is consistent with the presence of well-developed cotyledons, rich in reserve, which is a characteristic of this family (Subbarao 1939, Giulietti 1971, Lorenzo 1981; Souto & Oliveira 2005). The anatomical analysis of B. basiloba seeds showed the accumulation of proteins and lipids in the cotyledon and tegument. Malpighia glabra L. also accumulate these compounds in the same organs (Nacif et al. 1996). On the other hand, B. intermedia (Souto & Oliveira 2005), Banisteriopsis and Diplopterys pubipetala (A.Juss.) W.R. Anderson & C. Davis (Souto & Oliveira 2008) have as reserve substances lipids and starch. Seeds of Smilax polyantha Griseb., also a Cerrado species, store proteins and lipids (Martins et al. 2012). However, during germination process, S. polyantha synthesizes and accumulates starch in the cotyledons, which takes place concomitantly with the thinning of the endosperm cell walls (Martins et al. 2012). This fact indicated that, during germination, cell wall polysaccharides may be used for starch synthesis in this plant. Furthermore, according to the authors, S. polyantha do not use the seed protein content during germination. This process may not be the case of B. basiloba. Although neither starch nor protein contents were not analyzed, after germination, more precisely around the 60th day of cultivation, there was no visual of granules of any sort in the cotyledonary cells (data not shown).
According to Barroso et al. (1999), the embryo of the Byrsonima was axial and placed in the central region of the seed. Besides, these authors postulated that the hypocotyl- root system has no clear delimitation and the cotyledons are circinate and curled up in a spiral shape. Furthermore, Malpighiaceae embryos can be morphologically variable as seen in Malpighia glabra, Lophanthera lactescens Ducke and B. intermedia, which has straight, or folded, or coiled embryos (Nacif et al. 1996, Paoli 1997, Souto & Oliveira 2005). In B. basiloba embryos, although subtle, it was possible to differentiate the shoot/root promeristem, protoderm, procambium and ground meristem. Moreover, the data presented here showed that it was possible to differentiate between the hypocotyl and root transition zone. The Figure 14 clearly shows the existence of a small constriction, which is a morphological milestone to distinguish hypocotyl from root system (Souza et al. 2009).
In B. basiloba seedlings were noticeable that beside the promeristem, the in the leaf primordia, there was an epidermis with trichomes and a procambium with a partially differentiated vascular system. It appears that the differentiation of the tissues in this species occurs early. This fact certainly helps seedling establishment after germination.
Araújo (1994) and Oliveira (2001) stated that the morphology of cotyledons may vary among species of the same genus, which could facilitate the identification of seedlings a given species. Hence, as a comparison, the cotyledons of B. coriaceae (Sw.) DC. are lanceolate (Duke 1969), while B. basiloba have cotyledons oblong with a decurrent basis. Moreover, B. basiloba cotyledons remained attached to the seedling for more than six months and became photosynthetically active. As a result, the morpho- anatomical structure of the cotyledons after two months was very similar to the eophylls. These changes in B. basiloba cotyledons after the germination may have taxonomic applications, helping to recognize seedlings in the field.
In the Malpighiaceae there are hypostomatic and amphistomatic species, and, the paracytic stoma is the most frequent in this family (Beiguelman 1962, Giulietti 1971, Mamede 1993). The ecological advantages of amphistomy or hypostomy have not yet been fully understood (Palhares & Zaidan 2010, Palhares et al. 2011). Apparently, amphistomy occurs most frequently in sunny environments that are neither very dry nor very humid, such as the Cerrado. However, in similar ecosystems are found both hypostomatic and amphistomatic species within the same genus, which has brought new issues to be discussed about this topic (Palhares et al. 2011). With respect to eophylls, in B. basiloba, they have similar mesophyll organization as leaves from adult plants. Nevertheless, B. basiloba eophylls were amphistomatic while the adult leaves had stomata only on the abaxial side (Silveira, personal communication).
The apical bud of B. basiloba was exposed before the completion of eophyll elongation. Besides, densely haired eophylls are a common characteristic in Malpighiaceae (Araújo et al. 2010). In the current studied species, long trichomes were also seen in the leaf primordia and druses in the mesophyll. The function of trichomes in leaf primordia is to protect the apical bud. The trichome, generally, also contains phenolic compounds that protect against plagues and diseases, delimiting the development of pathogenic micro-organisms and predation by herbivores (Taiz & Zeiger 1998).
In the root-stem transition zone, which originated the xylopodium seen in adult plants, did not show any change in color and was oblique to the stem axis. Typically, during the xylopodium ontogenesis, the thickening of parenchymatic tissue occurs due to improvement in cell numbers and volume (Paviani 1987, Vilhalva & Appezzato-da-Glória 2006). This data is consistent with the findings of B. basiloba xylopodium development. Therefore, this fact has obvious applications for the propagation of this species. The natural ability of xylopodia to form new buds gives the root-stem transition zone an enormous potential to be used as explant source for the production of new plants, in nature as well as through agricultural techniques (Silveira et al., 2013b)
In conclusion, the anatomical description presented here can help with taxonomic identification of the species as well as to subsidize in vitro cultivation protocols. It is important to know the normal morpho-anatomical structure of seedlings to facilitate their recognition in the field, as well as determine the degree of normality of plants produced by in vitro procedures.