Analysis of peroxiporin-mediated H2O2 fluxes in HeLa cellular membrane: A therapeutic potential in redox signaling disorders
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Soria Sánchez, Paula Michelle; Núñez de Arenas, Arévalo; Sorrentino, Ilaria; Molina Oviedo, Angie Katherine; Medraño Fernández, IriaÁrea de conocimiento
Tecnología ElectrónicaPatrocinadores
This project has been developed in the UC3M facility core CleanRooms for Bioengineering. Financial support was provided by the Madrid Government (Comunidad de Madrid) under the Multiannual Agreement with UC3M in the line of ”Research Funds for Beatriz Galindo Fellowships” (REDOXSKIN-CM-UC3M), and in the context of the V PRICIT (Regional Programme of Research and Technological Innovation”, and from “Proyectos de I + D + I” (PID2020-114230 GA-I00) funded by MCIN/AEI/10.13039/501100011033Fecha de publicación
2023-11Editorial
Universidad Politécnica de CartagenaCita bibliográfica
SORIA, P. M. et al. Analysis of peroxiporin-mediated H2O2 fluxes in HeLa cellular membrane: A therapeutic potential in redox signaling disorders. En: XLI Congreso Anual de la Sociedad Española de Ingeniería Biomédica. Cartagena: Universidad Politécnica de Cartagena, 2023. Pp. 638-641. ISBN: 978-84-17853-76-1Palabras clave
PeroxiporinMembers of the family of aquaporin (AQP)
HeLa cells cytosol (HeLa HyPerCyto)
Endoplasmic reticulum (ER)
Resumen
Once considered merely a byproduct of oxidative respiration with deleterious effects, hydrogen peroxide (H2O2) has now emerged as a pivotal second messenger influencing celular processes by modulating protein function and gene expression. A particularity of the mechanism of action of this molecule resides in its compartmentalized production within cells. To reach its targets, H2O2 must cross cellular membranes aided by members of the family of aquaporin (AQP) channels. In HeLa cells, it has been demonstrated that AQP8 is the unique transporter mediating H2O2 internalization upon an exogenous challenge, despite the presence of abundant mRNA corresponding to AQP3. In this study, we have utilized the genetically encoded H2O2-specific ratiometric HyPer probe stably expressed in HeLa cells cytosol (HeLa HyPerCyto) to explore this paradox by measuring and characterizing H2O2 permeability across both channels. Live imaging analyses revealed that AQP8 is the transporter preferentially used at ...
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