Abstract
Photosynthetic microbial fuel cell (PMFC) is a novel technology, which employs organic pollutants and organisms to produce electrons and biomass and capture CO2 by bio-reactions. In this study, a new PMFC was developed based on Synechococcus sp. as a biocathode, and dairy wastewater was used in the anode chamber. Different experiments including batch feed mode, semi-continuous feed mode, Synechococcus feedstock to the anode chamber, Synechococcus-Chlorella mixed system, the feedstock of treated wastewater to the cathode chamber, and use of extra nutrients in the anodic chamber were performed to investigate the behavior of the PMFC system. The results indicated that the PMFC with a semi-continuous feed mode is more effective than a batch mode for electricity generation and pollutant removal. Herein, maximum power density, chemical oxygen demand removal, and Coulombic efficiency were 6.95 mW/m2 (450 Ω internal resistance), 62.94, and 43.16%, respectively, through mixing Synechococcus sp. and Chlorella algae in the batch-fed mode. The maximum nitrate and orthophosphate removal rates were 98.83 and 68.5%, respectively, wherein treated wastewater in the anode was added to the cathode. No significant difference in Synechococcus growth rate was found between the cathodic chamber of PMFC and the control cultivation cell. The heating value of the biocathode biomass at maximum Synechococcus growth rate (adding glucose into the anode chamber) was 0.2235 MJ/Kg, indicating the cell’s high ability for carbon dioxide recovery. This study investigated not only simultaneous bioelectricity production and dairy wastewater in a new PMFC using Synechococcus sp. but also studied several operational parameters and presented useful information about their effect on PMFC performance.
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References
Angelaa Lincy MJ, Ashok Kumar B, Vasantha VS, Varalakshmi P (2015) Microbial fuel cells: a promising alternative energy source. Bioenergy Oppor Challenges 62–85. https://doi.org/10.1201/b18718
Arun S, Sinharoy A, Pakshirajan K, Lens PNL (2020) Algae based microbial fuel cells for wastewater treatment and recovery of value-added products. Renew Sustain Energy Rev 132:110041. https://doi.org/10.1016/j.rser.2020.110041
Bazdar E, Roshandel R, Yaghmaei S, Mardanpour MM (2018) The effect of different light intensities and light/dark regimes on the performance of photosynthetic microalgae microbial fuel cell. Bioresour Technol 261:350–360. https://doi.org/10.1016/j.biortech.2018.04.026
Bazrafshan E, Moein H, Kord Mostafapour F, Nakhaie S (2013) Application of electrocoagulation process for dairy wastewater treatment. J Chem 2013:7–10. https://doi.org/10.1155/2013/640139
Bennetto HP (2016) Electricity Generation by Microorganisms. Butsuri 71:296–301. https://doi.org/10.11316/butsuri.71.5_296
Choudhury P, Prasad Uday US, Bandyopadhyay TK et al (2017a) Performance improvement of microbial fuel cell (MFC) using suitable electrode and Bioengineered organisms: a review. Bioeng 8:471–487. https://doi.org/10.1080/21655979.2016.1267883
Choudhury P, Shankar U, Uday P et al (2017b) Performance improvement of microbial fuel cells for waste water treatment along with value addition : a review on past achievements and recent perspectives. Renew Sustain Energy Rev 79:372–389. https://doi.org/10.1016/j.rser.2017.05.098
Colombo A, Marzorati S, Lucchini G et al (2017) Assisting cultivation of photosynthetic microorganisms by microbial fuel cells to enhance nutrients recovery from wastewater. Bioresour Technol 237:240–248. https://doi.org/10.1016/j.biortech.2017.03.038
Commault AS, Laczka O, Siboni N et al (2017) Electricity and biomass production in a bacteria-Chlorella based microbial fuel cell treating wastewater. J Power Sources 356:299–309. https://doi.org/10.1016/j.jpowsour.2017.03.097
Elmekawy A, Hegab HM, Vanbroekhoven K, Pant D (2014) Techno-productive potential of photosynthetic microbial fuel cells through different configurations. Renew Sustain Energy Rev 39:617–627. https://doi.org/10.1016/j.rser.2014.07.116
Fan Y, Hu H, Liu H (2007) Enhanced Coulombic efficiency and power density of air-cathode microbial fuel cells with an improved cell configuration. J Power Sources 171:348–354. https://doi.org/10.1016/j.jpowsour.2007.06.220
Gadhamshetty V, Belanger D, Gardiner CJ et al (2013) Evaluation of Laminaria-based microbial fuel cells (LbMs) for electricity production. Bioresour Technol 127:378–385. https://doi.org/10.1016/j.biortech.2012.09.079
Gonzalez Del Campo A, Perez JF, Cañizares P et al (2015) Characterization of light/dark cycle and long-term performance test in a photosynthetic microbial fuel cell. Fuel 140:209–216. https://doi.org/10.1016/j.fuel.2014.09.087
Gude VG (2016) Wastewater treatment in microbial fuel cells e an overview. J Clean Prod 122:287–307. https://doi.org/10.1016/j.jclepro.2016.02.022
Hu X, Liu B, Zhou J et al (2015) CO2 Fixation, lipid production, and power generation by a novel air-lift-type microbial carbon capture cell system. Environ Sci Technol 49:10710–10717. https://doi.org/10.1021/acs.est.5b02211
Huang Y, Huang Y, Liao Q et al (2017) Improving phosphorus removal efficiency and Chlorella vulgaris growth in high-phosphate MFC wastewater by frequent addition of small amounts of nitrate. Int J Hydrogen Energy 42:27749–27758. https://doi.org/10.1016/j.ijhydene.2017.05.069
Ieropoulos I, Winfield J, Greenman J (2010) Effects of flow-rate, inoculum and time on the internal resistance of microbial fuel cells. Bioresour Technol 101:3520–3525. https://doi.org/10.1016/j.biortech.2009.12.108
Jadhav DA, Neethu B, Ghangrekar MM (2019) Microbial carbon capture cell: advanced bio-electrochemical system for wastewater treatment, electricity generation and algal biomass production. Appl Microalgae Wastewater Treat 317–338. https://doi.org/10.1007/978-3-030-13909-4_14
Juang DF, Lee CH, Hsueh SC (2012) Comparison of electrogenic capabilities of microbial fuel cell with different light power on algae grown cathode. Bioresour Technol 123:23–29. https://doi.org/10.1016/j.biortech.2012.07.041
Kakarla R, Min B (2019) Sustainable electricity generation and ammonium removal by microbial fuel cell with a microalgae assisted cathode at various environmental conditions. Bioresour Technol 284:161–167. https://doi.org/10.1016/j.biortech.2019.03.111
Karthikeyan R, Selvam A, Cheng KY, Wong JWC (2016) Influence of ionic conductivity in bioelectricity production from saline domestic sewage sludge in microbial fuel cells. Bioresour Technol 200:845–852. https://doi.org/10.1016/j.biortech.2015.10.101
Katuri KP, Scott K, Head IM et al (2011) Microbial fuel cells meet with external resistance. Bioresour Technol 102:2758–2766. https://doi.org/10.1016/j.biortech.2010.10.147
Kelly PT, He Z (2014) Bioresource Technology Nutrients removal and recovery in bioelectrochemical systems : a review. Bioresour Technol 153:351–360. https://doi.org/10.1016/j.biortech.2013.12.046
Khandelwal A, Vijay A, Dixit A, Chhabra M (2018) Microbial fuel cell powered by lipid extracted algae: a promising system for algal lipids and power generation. Bioresour Technol 247:520–527. https://doi.org/10.1016/j.biortech.2017.09.119
Li M, Zhou M, Luo J et al (2019) Carbon dioxide sequestration accompanied by bioenergy generation using a bubbling-type photosynthetic algae microbial fuel cell. Bioresour Technol 280:95–103. https://doi.org/10.1016/j.biortech.2019.02.038
Lobato J, González del Campo A, Fernández FJ et al (2013) Lagooning microbial fuel cells: A first approach by coupling electricity-producing microorganisms and algae. Appl Energy 110:220–226. https://doi.org/10.1016/j.apenergy.2013.04.010
Ma J, Wang Z, Zhang J et al (2017) Cost-effective Chlorella biomass production from dilute wastewater using a novel photosynthetic microbial fuel cell (PMFC). Water Res 108:356–364. https://doi.org/10.1016/j.watres.2016.11.016
Ma M, Cao L, Chen L et al (2015a) <B>A carbon-neutral photosynthetic microbial fuel cell powered by</B> <B><I>Microcystis aeruginosa</I></B>. Water Environ Res 87:644–649. https://doi.org/10.2175/106143015x14212658614883
Ma M, You S, Gong X et al (2015b) Silver / iron oxide / graphitic carbon composites as bacteriostatic catalysts for enhancing oxygen reduction in microbial fuel cells. J Power Sources 283:74–83. https://doi.org/10.1016/j.jpowsour.2015.02.100
Manchanda T, Tyagi R, Nalla VK, et al (2018) Power generation by algal microbial fuel cell along with simultaneous treatment of sugar industry wastewater. J Bioprocess Biotech 08 https://doi.org/10.4172/2155-9821.1000323
Mayers JJ, Flynn KJ, Shields RJ (2014) Influence of the N: P supply ratio on biomass productivity and time-resolved changes in elemental and bulk biochemical composition of Nannochloropsis sp. Bioresour Technol 169:588–595. https://doi.org/10.1016/j.biortech.2014.07.048
Mekuto L, Olowolafe AVA, Pandit S et al (2020) Microalgae as a biocathode and feedstock in anode chamber for a self-sustainable microbial fuel cell technology: a review. S Afr J Chem Eng 31:7–16. https://doi.org/10.1016/j.sajce.2019.10.002
Min B, Román ÓB, Angelidaki I (2008) Importance of temperature and anodic medium composition on microbial fuel cell (MFC) performance. Biotechnol Lett 30:1213–1218. https://doi.org/10.1007/s10529-008-9687-4
Mohamed SN, Hiraman PA, Muthukumar K, Jayabalan T (2020) Bioresource technology bioelectricity production from kitchen wastewater using microbial fuel cell with photosynthetic algal cathode. Bioresour Technol 295:122226. https://doi.org/10.1016/j.biortech.2019.122226
Mohan SV, Srikanth S, Chiranjeevi P et al (2014) Bioresource technology algal biocathode for in situ terminal electron acceptor ( TEA ) production : synergetic association of bacteria – microalgae metabolism for the functioning of biofuel cell. Bioresour Technol 166:566–574. https://doi.org/10.1016/j.biortech.2014.05.081
Naina Mohamed S, Ajit Hiraman P, Muthukumar K, Jayabalan T (2020) Bioelectricity production from kitchen wastewater using microbial fuel cell with photosynthetic algal cathode. Bioresour Technol 295:122226. https://doi.org/10.1016/j.biortech.2019.122226
Nam JY, Kim HW, Lim KH et al (2010) Variation of power generation at different buffer types and conductivities in single chamber microbial fuel cells. Biosens Bioelectron 25:1155–1159. https://doi.org/10.1016/j.bios.2009.10.005
Nayak JK, Ghosh UK (2019) Post treatment of microalgae treated pharmaceutical wastewater in photosynthetic microbial fuel cell (PMFC) and biodiesel production. Biomass and Bioenergy 131. https://doi.org/10.1016/j.biombioe.2019.105415
Nguyen HTH, Min B (2020) Leachate treatment and electricity generation using an algae-cathode microbial fuel cell with continuous flow through the chambers in series. Elsevier B.V
Nien PC, Lee CY, Ho KC et al (2011) Power overshoot in two-chambered microbial fuel cell (MFC). Bioresour Technol 102:4742–4746. https://doi.org/10.1016/j.biortech.2010.12.015
Papaharalabos G, Greenman J, Melhuish C, Ieropoulos I (2015) ScienceDirect A novel small scale Microbial Fuel Cell design for increased electricity generation and waste water treatment. Int J Hydrogen Energy 40:4263–4268. https://doi.org/10.1016/j.ijhydene.2015.01.117
Pei H, Yang Z, Nie C et al (2018) Using a tubular photosynthetic microbial fuel cell to treat anaerobically digested effluent from kitchen waste: mechanisms of organics and ammonium removal. Bioresour Technol 256:11–16. https://doi.org/10.1016/j.biortech.2018.01.144
Pham TH, Aelterman P, Verstraete W (2009) Bioanode performance in bioelectrochemical systems: recent improvements and prospects. Trends Biotechnol 27:168–178. https://doi.org/10.1016/j.tibtech.2008.11.005
Pisciotta JM, Zou Y, Baskakov I V (2011) Role of the photosynthetic electron transfer chain in electrogenic activity of cyanobacteria. 377–385. https://doi.org/10.1007/s00253-011-3239-x
Rashid N, Cui YF, Muhammad SUR, Han JI (2013) Enhanced electricity generation by using algae biomass and activated sludge in microbial fuel cell. Sci Total Environ 456–457:91–94. https://doi.org/10.1016/j.scitotenv.2013.03.067
Saba B, Christy AD, Yu Z, Co AC (2017) Sustainable power generation from bacterio-algal microbial fuel cells ( MFCs ): an overview. Renew Sustain Energy Rev 73:75–84. https://doi.org/10.1016/j.rser.2017.01.115
Saha P, Amanullah S, Dey A (2020) Electrocatalytic reduction of nitrogen to hydrazine using a trinuclear nickel complex. J Am Chem Soc 142:17312–17317. https://doi.org/10.1021/jacs.0c08785
Samal K, Dash RR, Bhunia P (2018) Design and development of a hybrid macrophyte assisted vermifilter for the treatment of dairy wastewater: a statistical and kinetic modelling approach. Sci Total Environ 645:156–169. https://doi.org/10.1016/j.scitotenv.2018.07.118
Sarkar B, Chakrabarti PP, Vijaykumar A, Kale V (2006) Wastewater treatment in dairy industries - possibility of reuse. Desalination 195:141–152. https://doi.org/10.1016/j.desal.2005.11.015
Shukla M, Kumar S (2018) Algal growth in photosynthetic algal microbial fuel cell and its subsequent utilization for biofuels. Renew Sustain Energy Rev 82:402–414. https://doi.org/10.1016/j.rser.2017.09.067
Slavov AK (2017) General characteristics and treatment possibilities of dairy wastewater -a review. Food Technol Biotechnol 55:14–28. https://doi.org/10.17113/ftb.55.01.17.4520
Srivastava P, Gupta S, Garaniya V et al (2019) Up to 399 mV bioelectricity generated by a rice paddy-planted microbial fuel cell assisted with a blue-green algal cathode. Environ Chem Lett 17:1045–1051. https://doi.org/10.1007/s10311-018-00824-2
Strik DPBTB, Timmers RA, Helder M et al (2011) Microbial solar cells: applying photosynthetic and electrochemically active organisms. Trends Biotechnol 29:41–49. https://doi.org/10.1016/j.tibtech.2010.10.001
Uggetti E, Puigagut J (2016) Photosynthetic membrane-less microbial fuel cells to enhance microalgal biomass concentration. Bioresour Technol 218:1016–1020. https://doi.org/10.1016/j.biortech.2016.07.062
Venkata Mohan S, Srikanth S, Chiranjeevi P et al (2014) Algal biocathode for in situ terminal electron acceptor (TEA) production: synergetic association of bacteria-microalgae metabolism for the functioning of biofuel cell. Bioresour Technol 166:566–574. https://doi.org/10.1016/j.biortech.2014.05.081
Venkata Subhash G, Chandra R, Venkata Mohan S (2013) Microalgae mediated bio-electrocatalytic fuel cell facilitates bioelectricity generation through oxygenic photomixotrophic mechanism. Bioresour Technol 136:644–653. https://doi.org/10.1016/j.biortech.2013.02.035
Winfield J, Ieropoulos I, Greenman J, Dennis J (2011) The overshoot phenomenon as a function of internal resistance in microbial fuel cells. Bioelectrochem 81:22–27. https://doi.org/10.1016/j.bioelechem.2011.01.001
Wu Y-C, Wang Z, jie, Zheng Y, et al (2014) Light intensity affects the performance of photo microbial fuel cells with Desmodesmus sp. A8 as cathodic microorganism. Appl Energy 116:86–90. https://doi.org/10.1016/j.apenergy.2013.11.066
Xiao L, Young EB, Berges JA, He Z (2012) Integrated photo-bioelectrochemical system for contaminants removal and bioenergy production 46:11459–11466 . https://doi.org/10.1021/es303144n
Xu D, Savage PE (2018)Supercritical water upgrading of water-insoluble and water-soluble biocrudes from hydrothermal liquefaction of Nannochloropsis microalgae. J Supercrit Fluids 133:683–689. https://doi.org/10.1016/j.supflu.2017.07.016
Yadav AK, Nayak SK, Acharya BC, Mishra BK (2015) Algal-assisted microbial fuel cell for wastewater treatment and bioelectricity generation. Energy Sources, Part A Recover Util Environ Eff 37:127–133. https://doi.org/10.1080/15567036.2011.576422
Yagishita T, Sawayama S, Tsukahara K, Ogi T (1996) Photosynthetic bio-fuel cells using cyanobacteria. Renew Energy 9:958–961. https://doi.org/10.1016/0960-1481(96)88439-4
Yang Z, Nie C, Hou Q et al (2019) Coupling a photosynthetic microbial fuel cell (PMFC) with photobioreactors (PBRs) for pollutant removal and bioenergy recovery from anaerobically digested effluent. Chem Eng J 359:402–408. https://doi.org/10.1016/j.cej.2018.11.136
Yang Z, Pei H, Hou Q et al (2018) Algal bio fi lm-assisted microbial fuel cell to enhance domestic wastewater treatment : nutrient, organics removal and bioenergy production. Chem Eng J 332:277–285. https://doi.org/10.1016/j.cej.2017.09.096
Zhang Y, Noori JS, Angelidaki I (2011) Simultaneous organic carbon, nutrients removal and energy production in a photomicrobial fuel cell (PFC). Energy Environ Sci 4:4340–4346. https://doi.org/10.1039/c1ee02089g
Zhang Y, Zhao Y, Zhou M (2019) A photosynthetic algal microbial fuel cell for treating swine wastewater. Environ Sci Pollut Res 26:6182–6190. https://doi.org/10.1007/s11356-018-3960-4
Zheng S, Yang F, Chen S, et al (2015) Binder-free carbon black / stainless steel mesh composite electrode for high-performance anode in microbial fuel cells. 284:252–257. https://doi.org/10.1016/j.jpowsour.2015.03.014
Zhou M, He H, Jin T, Wang H (2012) Power generation enhancement in novel microbial carbon capture cells with immobilized Chlorella vulgaris. J Power Sources 214:216–219. https://doi.org/10.1016/j.jpowsour.2012.04.043
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The authors would like to thank the authorities of the University of Tehran for technical and financial support.
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This research was supported by the Faculty of Environment and the Green Technology Laboratory (GTL) at the School of Chemical Engineering, University of Tehran.
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Sahar Khodadi: conceptualization, methodology, validation, formal analysis, investigation, resources, data curation, writing—original draft.
Abdolreza Karbassi: conceptualization, methodology, validation, formal analysis, resources, data curation, writing—original draft.
Omid Tavakoli: conceptualization, methodology, validation, formal analysis, investigation, resources.
Majid Baghdadi: conceptualization, validation, formal analysis, investigation, resources, data curation, writing—original draft.
Zeinab Zare: validation, formal analysis, investigation, data curation, writing—original draft.
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Khodadi, S., Karbassi, A., Tavakoli, O. et al. Simultaneous dairy wastewater treatment and bioelectricity production in a new microbial fuel cell using photosynthetic Synechococcus. Int Microbiol 26, 741–756 (2023). https://doi.org/10.1007/s10123-023-00328-2
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DOI: https://doi.org/10.1007/s10123-023-00328-2