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Study on effects of miR-141-3p in proliferation, migration, invasion and apoptosis of colon cancer cells by inhibiting Bcl2

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Abstract

Purpose

This study aimed to investigate the relationship between miR-141-3p and B lymphocyte-2 gene (Bcl2) gene and its biological behavior on colon cancer cell line SW480.

Methods

qRT-PCR was used to detect the expression level of miR-141-3p in colon cancer tissues and adjacent tissues, as well as in colon cancer cell line and normal human colonic epithelial cell line FHC. MTT assay, wound assay, and Transwell demonstrated the effects of miR-141-3p on colon cancer proliferation, migration and invasion. Targetscan7.1 predictive software and dual luciferase reporter assays were used to detect the targeted regulation of miR-141-3p on the apoptosis-related gene Bcl2. MTT assay, wound assay, Transwell and flow cytometry were used to detect the effect of Bcl2 on miR-141-3p on colon cancer proliferation, migration, invasion and apoptosis.

Results

Compared with adjacent tissues, the expression of miR-141-3p in colon cancer tissues was significantly down-regulated. Colon cancer patients with low expression of miR-141-3p had poorer prognosis. Compared with normal colonic epithelial cells, miR-141-3p expression was significantly down-regulated in colon cancer cell lines, and overexpression of miR-141-3p significantly attenuated the proliferation, migration and invasion of colon cancer cells. Knockdown of miR-141-3p significantly promoted the proliferation, migration and invasion of colon cancer cells. miR-141-3p targets the negative regulation of Bcl2. Knockdown of Bcl2 significantly attenuated the promotion of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells and inhibition of apoptosis. Knockdown of Bcl2 significantly enhanced the inhibition effect of miR-141-3p inhibitor on proliferation, migration and invasion of colon cancer cells.

Conclusions

In conclusion, miR-141-3p can inhibit the cancer by regulating Bcl2, and miR-141-3p has the potential to become a potential therapeutic target for colon cancer.

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Funding

This work was supported by the Science and Technology Innovation Incentive Project of Qiqihar City (CSFGG-2020166).

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Authors and Affiliations

  1. Ward 1, Department of General Surgery, The Third Affiliated Hospital of Qiqihaer Medical University, No. 27 Taishun Street, Tiefeng District, Qiqihaer City, 161000, Heilongjiang Province, China

    S. J. Tong, H. F. Guo, J. Yang, Y. P. Qi & S. Lu

  2. Department of Stomatology, The Third Affiliated Hospital of Qiqihaer Medical University, Qiqihaer City, 161000, China

    X. Y. Zhang

Authors
  1. S. J. Tong
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  2. X. Y. Zhang
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  3. H. F. Guo
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  4. J. Yang
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  5. Y. P. Qi
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  6. S. Lu
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Contributions

ST and SL conceived and designed the study, and drafted the manuscript. ST, XZ, HG, JY and YQ collected, analyzed and interpreted the experimental data. XZ and SL revised the manuscript for important intellectual content. All authors read and approved the final manuscript.

Corresponding author

Correspondence to S. Lu.

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The authors declare that they have no conflict of interest.

Ethical approval

The study was approved by Ethical Committee of The Third Affiliated Hospital of Qiqihaer Medical University and conducted in accordance with the ethical standards.

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Tong, .J., Zhang, X.Y., Guo, H.F. et al. Study on effects of miR-141-3p in proliferation, migration, invasion and apoptosis of colon cancer cells by inhibiting Bcl2. Clin Transl Oncol 23, 2526–2535 (2021). https://doi.org/10.1007/s12094-021-02653-2

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  • DOI: https://doi.org/10.1007/s12094-021-02653-2

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