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Dietary flaxseed oil and fish oil ameliorates renal oxidative stress, protein glycation, and inflammation in streptozotocin–nicotinamide-induced diabetic rats

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Abstract

Protective and prophylactic effects of omega-3 fatty acids on oxidative stress and inflammation are well known. We assessed beneficial effects of flaxseed oil and fish oil on streptozotocin (65 mg/kg; i.p.)–nicotinamide (110 mg/kg; i.p.) induced diabetic rats by studying renal expression of antioxidant and inflammatory genes. Diabetic rats given 10 % flaxseed oil or 10 % fish oil diet for 35 days showed significant decrease in renal lipid peroxidation. Flaxseed oil diet resulted in up-regulation of renal superoxide dismutase-1 (SOD-1) (activity and expression) and glutathione peroxidase-1 (GPx-1) expression. Furthermore, both diets up-regulated catalase (CAT) (activity and expression) and down-regulated heme oxygenase-1 (HO-1) expression. Both diets were able to limit the renal advanced glycation end products (AGEs) formation and reduced receptor of AGE (RAGE) protein expression significantly. Expressions of interleukin-6 (IL-6) and NF-κB p65 subunit were down-regulated significantly by flaxseed oil or fish oil diet. The histological tubular injuries were also lowered by both diets. These results suggest that dietary ω-3 fatty acids may slow the progression of diabetic nephropathy (DN) associated with oxidative stress, glycation, and inflammation in the kidney.

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Acknowledgments

We acknowledge the Department of Biotechnology (DBT), Government of India, for providing financial support.

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Authors and Affiliations

  1. Department of Pharmaceutical Biotechnology, Bharati Vidyapeeth Deemed University, Poona College of Pharmacy, Erandwane, Pune, Maharashtra, 411038, India

    Nivedita M. Jangale & Abhay M. Harsulkar

  2. Interactive Research School for Health Affairs, Bharati Vidyapeeth Deemed University, Pune, Maharashtra, 411043, India

    Prasad P. Devarshi

  3. Proteomics Facility, Division of Biochemical Sciences, CSIR-National Chemical Laboratory, Pune, Maharashtra, 411008, India

    Sneha B. Bansode & Mahesh J. Kulkarni

Authors
  1. Nivedita M. Jangale
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  2. Prasad P. Devarshi
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  3. Sneha B. Bansode
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  4. Mahesh J. Kulkarni
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  5. Abhay M. Harsulkar
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Correspondence to Abhay M. Harsulkar.

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Jangale, N.M., Devarshi, P.P., Bansode, S.B. et al. Dietary flaxseed oil and fish oil ameliorates renal oxidative stress, protein glycation, and inflammation in streptozotocin–nicotinamide-induced diabetic rats. J Physiol Biochem 72, 327–336 (2016). https://doi.org/10.1007/s13105-016-0482-8

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  • DOI: https://doi.org/10.1007/s13105-016-0482-8

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