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Effects of treadmill running and rutin on lipolytic signaling pathways and TRPV4 protein expression in the adipose tissue of diet-induced obese mice

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Abstract

To explore the effects of rutin and exercise on high-fat diet (HFD)-induced disrupted lipolytic signaling, adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) signaling, transient receptor potential cation channel subfamily V member 4 (TRPV4) and its associated protein expression, and whether depot-specific effects existed. C57BL/6J mice were randomized into five groups: chow group, HFD, HFD plus rutin intervention group (HR), HFD combined with treadmill running group (HE), and HFD combined with treadmill running and rutin intervention group (HRE). At the end of the 16-week intervention, lipolytic markers, AMPK signaling pathways, TRPV4, and peroxisome proliferator-activated receptor gamma coactivator 1α + β (PGC-1α + β) from adipose tissue were measured by western blotting. In epididymal adipose tissue, HFD resulted in significant reduction in the phosphorylation of hormone sensitive lipase at serine660 (p-HSL660), perilipin A, phosphoenolpyruvate carboxykinase (PEPCK), p-AMPK, and p-acetyl-CoA carboxylase (ACC) protein expression. Exercise intervention and exercise plus rutin completely restored p-HSL660, perilipin A, PEPCK, p-AMPK, and p-ACC protein expression to normal level. HFD and HR groups have reduced expression of PGC-1α + β, exercise, and exercise plus rutin completely restored PGC-1α + β expression to normal level. In subcutaneous adipose tissue, HFD elevated TRPV4, exercise, and exercise plus rutin completely reduced TRPV4 to normal level. HR, HE, and HRE group have increased PGC-1α + β. In conclusion, depot-specific effects existed in regards to how rutin and exercise affect lipolytic signaling and p-AMPK, as well as TRPV4 and PGC-1α + β expression.

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Acknowledgments

This study is supported by the Natural Science Foundation of China (grant no. 81472975 to Wan Z. and grant no. 81273067 to Qin L.) and Jiangsu Province Preventive Medical Program “Establishing the system of all of the subtype complete genome of the influenza: a virus” (program no. Y2012008).

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Authors and Affiliations

  1. Department of Nutrition and Food Hygiene, School of Public Health, Soochow University, Renai Road 199, Dushuhu Higher Education Town, Suzhou, China, 215123

    Neng Chen, Jinbo Cheng, Lingmei Zhou, Lihua Chen, Liqiang Qin & Zhongxiao Wan

  2. Suzhou Industrial Park Center Disease Control and Prevention, 58 Suqian Road, Suzhou, 215123, People’s Republic of China

    Ting Lei

  3. Suzhou Center for Disease Control and Prevention, 72 Sanxiang Road, 215004, Suzhou, People’s Republic of China

    Qiang Shen

  4. Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Disease, Soochow University, 199 Renai Road, Suzhou, 215123, People’s Republic of China

    Zhongxiao Wan

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  1. Neng Chen
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  2. Jinbo Cheng
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  3. Lingmei Zhou
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  5. Lihua Chen
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Correspondence to Liqiang Qin or Zhongxiao Wan.

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Chen, N., Cheng, J., Zhou, L. et al. Effects of treadmill running and rutin on lipolytic signaling pathways and TRPV4 protein expression in the adipose tissue of diet-induced obese mice. J Physiol Biochem 71, 733–742 (2015). https://doi.org/10.1007/s13105-015-0437-5

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  • DOI: https://doi.org/10.1007/s13105-015-0437-5

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